Kinetic Analysis of Gluconacetobacter diazotrophicus Cultivated on a Bench Scale: Modeling the Effect of pH and Design of a Sucrose-Based Medium

Author:

Restrepo Gloria M.1ORCID,Rincón Alejandro12ORCID,Sánchez Óscar J.3ORCID

Affiliation:

1. Research Group on Microbiology and Agro-Industrial Biotechnology (GIMIBAG), Research Institute in Microbiology and Agro-Industrial Biotechnology, Universidad Católica de Manizales, Carrera 23 No. 60-63, Manizales 170002, Colombia

2. Research Group on Technological and Environmental Developments (GIDTA), Faculty of Engineering and Architecture, Universidad Católica de Manizales, Carrera 23 No. 60-63, Manizales 170002, Colombia

3. Center for Technological Development—Bioprocess and Agro-Industry Plant, Department of Engineering, Universidad de Caldas, Calle 65 No. 26-10, Manizales 170004, Colombia

Abstract

Gluconacetobacter diazotrophicus is an endophytic bacterium that has shown important plant growth-promoting properties. During the growth of G. diazotrophicus with high carbon source concentrations, organic acids are produced, and pH decreases, thus inhibiting biomass growth. The objective of this work was to design a sucrose-based medium and perform a kinetic analysis of the batch submerged cultivation of this bacterium in a 3 L stirred-tank bioreactor without pH control. A mathematical model was proposed for representing G. diazotrophicus concentration, considering the inhibitory effect of hydrogen ion concentration. It comprises a biomass growth model, a specific growth rate expression that accounts for the inhibitory effect of hydrogen concentration, and a hydrogen model that represents the relationship between hydrogen and biomass concentrations. The sucrose-based medium proved its suitability for G. diazotrophicus growth. A higher biomass concentration (1.10 g/L) was obtained in a modified LGI-P medium containing 30 g/L sucrose with a three-fold increase in biomass production relative to the initial inoculation. The model allowed a satisfactory description of the experimental data obtained, and it could be used to design a cultivation strategy to maximize biomass production leading to the production of an alternative microbial inoculant for plant growth promotion of economically important crops.

Funder

Colombian Ministry of Science, Technology, and Innovation, Minciencias

Research Direction at the Universidad Católica de Manizales

Vice-Rectorate of Research and Graduate Studies at the Universidad de Caldas

Universidad de Caldas

Evaluation of an alternative liquid medium for the production of biomass of Gluconacetobacter diazotrophicus GIBI025

Publisher

MDPI AG

Subject

Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Food Science

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