Affiliation:
1. College of Life Science, Northeast Agricultural University, Harbin 150030, China
2. Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
3. School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China
Abstract
Artemisia annua residue (ARR) is a pharmaceutical by-product produced after the extraction of artemisinin; it is rich in protein, crude fat, vitamins, trace elements, and bioactive compounds and contains negligible anti-nutritional factors. The present study aimed to optimize the fermentation conditions of ARR, evaluate the compound and microbial compositions of fermented AAR, and explore its effects on the production performance of laying hens. A total of 288 Xinyang black-feather laying hens were randomly allocated into four treatments for 30 days, including a control group (basal diet) and a basal diet supplemented with 1%, 2%, and 4% fermented AAR, respectively. The results showed that the optimized fermentation conditions of AAR were 80% moisture content, 3% inoculation quantity, 34 °C fermentation for 6 days, initial pH at 8, and 60 mesh (sieving). The compounds of 2-furyl-5-methyl furan, deoxyartemisinin, phytol, n-hexadecanoic acid, aromandendrene, and calarene had higher contents (average 6.86%) in the fermented AAR. The bacteria of Proteobacteria and Firmicutes (average 45.18%) were the most abundant phyla, and Acinetobacter, Bacillus, and Brevundimonas (average 15.87%) were the most abundant genera in the fermented AAR. The fungi of Phragmoplastophyta, Vertebrata, and Ascomycota (average 30.13%) were the most abundant phyla, and Magnoliophyta, Mammalia, Wickerhamomyces-Candida_clade, and Aspergillus were the most abundant genera (average 21.12%) in the fermented AAR. Furthermore, dietary supplementation of fermented AAR increased the average daily feed intake (ADFI), egg weight, and albumen height. Dietary supplementation of 2% and 4% fermented AAR increased the laying rate, while 2% fermented AAR increased the Haugh unit and decreased the feed-to-egg ratio. Collectively, it is concluded that fermented AAR has the potential to become a phytogenic feed additive, and dietary supplementation of 2% fermented AAR had better effects on the production performance of laying hens.
Funder
City-School Cooperation Project of the Special Funds of Science and Technology in Fuyang City undertaken by Fuyang Normal University
Special Funds of Construction of Innovative Provinces in Hunan Province
Project of Provincial Research Institutes of Scientific Research Business Fee in Heilongjiang Province
National Natural Science Foundation of China
Key R&D projects in Heilongjiang Province
Subject
Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Food Science
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