Affiliation:
1. Institute of Biomass Engineering, Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass, Key Laboratory of Energy Plants Resource and Utilization, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China
Abstract
Metal chloride salt pretreatment was performed to isolate and convert cellulose to glucose from poplar. A glucose yield of 82.0% ± 0.7 was achieved after 0.05 mol/L AlCl3 pretreatment conducted at 180 °C for 20 min, ascribing to the removal of hemicellulose, the alteration of crystallinity, surface morphology, and the retention of the majority of cellulose. Then, the influence of different additives on glucose yield was assessed, generating the highest glucose yield of 88.5 ± 0.06 with the addition of PEG 8000. Meanwhile, a similar glucose yield of 82.8% ± 0.3 could be obtained with PEG 8000 when hydrolysis time was reduced by a quarter and enzyme dosage by three-quarters. It can be seen that AlCl3 pretreatment is a viable and efficient pretreatment method for poplar, while the addition of PEG 8000 can enhance the enzymatic efficiency and reduce cellulase loading, ascribing to the reservation of free enzyme and enzyme activity in the supernatant and the reduction in surface tension, which provide an idea to improve the economics of the enzymatic conversion of poplar.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Subject
Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Food Science