Affiliation:
1. Liaoning Academy of Agriculture Sciences, Shenyang 110161, China
2. College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China
Abstract
To investigate the cold response mechanism and low temperature regulation of flowering in tulips, this study identified 32 MADS-box transcription factor family members in tulips based on full-length transcriptome sequencing, named TgMADS1-TgMADS32. Phylogenetic analysis revealed that these genes can be divided into two classes: type I and type II. Structural analysis showed that TgMADS genes from different subfamilies have a similar distribution of conserved motifs. Quantitative real-time PCR results demonstrated that some TgMADS genes (e.g., TgMADS3, TgMADS15, TgMADS16, and TgMADS19) were significantly upregulated in buds and stems under cold conditions, implying their potential involvement in the cold response of tulips. In summary, this study systematically identified MADS family members in tulips and elucidated their evolutionary relationships, gene structures, and cold-responsive expression patterns, laying the foundation for further elucidating the roles of these transcription factors in flowering and the cold adaptability of tulips.
Funder
Program of China Agriculture Research System
Subject Construction Program of Liaoning Academy of Agricultural Sciences
Presidential Foundation of Liaoning Academy of Agricultural Sciences
Subject
Genetics (clinical),Genetics
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