Role of Different Members of the AGPAT Gene Family in Milk Fat Synthesis in Bubalus bubalis
Author:
Li Zhipeng1ORCID, Li Ruijia1, Ren Honghe1, Qin Chaobin1, Su Jie1, Song Xinhui1, Wang Shuwan1, Liu Qingyou2, Liu Yang3, Cui Kuiqing2
Affiliation:
1. Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, China 2. Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528225, China 3. Guangxi Zhuang Autonomous Region Center for Analysis and Test Research, Nanning 530022, China
Abstract
During triacylglycerol synthesis, the acylglycerol-3-phosphate acyltransferase (AGPAT) family catalyzes the conversion of lysophosphatidic acid to phosphatidic acid and the acylation of sn-2 fatty acids. However, the catalytic activity of different AGPAT members is different. Therefore, this study aimed to investigate the mechanism through which different AGPATs affect the efficiency of TAG synthesis and fatty acid composition. The conservation of amino acid sequences and protein domains of the AGPAT family was analyzed, and the functions of AGPAT1, AGPAT3, and AGPAT4 genes in buffalo mammary epithelial cells (BMECs) were studied using RNA interference and gene overexpression. Prediction of the protein tertiary structure of the AGPAT family demonstrated that four conservative motifs (motif1, motif2, motif3, and motif6) formed a hydrophobic pocket in AGPAT proteins, except AGPAT6. According to cytological studies, AGPAT1, AGPAT3, and AGPAT4 were found to promote the synthesis and fatty acid compositions of triacylglycerol, especially UFA compositions of triacylglycerol, by regulating ACSL1, FASN, GPAM, DGAT2, and PPARG gene expression. This study provides new insights into the role of different AGPAT gene family members involved in TAG synthesis, and a reference for improving the fatty acid composition of milk.
Funder
Guangxi Science and Technology Major Project Guangxi Natural Science Foundation National Natural Science Foundation of China
Subject
Genetics (clinical),Genetics
Reference46 articles.
1. Li, Z., Lu, S., Cui, K., Shafique, L., Luo, C., Wang, Z., Ruan, J., Qian, Q., and Liu, Q. (2020). Fatty acid biosynthesis and transcriptional regulation of Stearoyl-CoA Desaturase 1 (SCD1) in buffalo milk. BMC Genet., 21. 2. Bubalus bubalis: A short story;Minervino;Front. Vet. Sci.,2020 3. Composition of milk from minor dairy animals and buffalo breeds: A biodiversity perspective;Medhammar;J. Sci. Food Agric.,2012 4. Dietary saturated fatty acid type impacts obesity-induced metabolic dysfunction and plasma lipidomic signatures in mice;Bukowski;J. Nutr. Biochem.,2019 5. Nie, P., Pan, B., Ahmad, M.J., Zhang, X., Chen, C., Yao, Z., Lv, H., Wei, K., and Yang, L. (2022). Summer buffalo milk produced in China: A desirable diet enriched in polyunsaturated fatty acids and amino acids. Foods, 11.
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