Identification of Differentially Expressed Genes after Endurance Runs in Karbadian Horses to Determine Candidates for Stress Indicators and Performance Capability
Author:
Reißmann Monika1ORCID, Rajavel Abirami2ORCID, Kokov Zaur A.3ORCID, Schmitt Armin O.24ORCID
Affiliation:
1. Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt-Universität zu Berlin, Unter den Linden 6, 10099 Berlin, Germany 2. Breeding Informatics Group, Department of Animal Sciences, Georg-August University, Margarethe von Wrangell-Weg 7, 37075 Göttingen, Germany 3. Institute of Physics and Mathematics, Kabardino-Balkarian State University, Chernyshevsky 173, Nalchik 360004, Russia 4. Center for Integrated Breeding Research (CiBreed), Georg-August University, Carl-Sprengel-Weg 1, 37075 Göttingen, Germany
Abstract
RNA sequencing makes it possible to uncover genetic mechanisms that underlie certain performance traits. In order to gain a deeper insight into the genetic background and biological processes involved in endurance performance in horses, the changes in the gene expression profiles induced by endurance runs over long (70 km) and short (15 km) distances in the blood of Kabardian horses (Equus caballus) were analyzed. For the long-distance runs, we identified 1484 up- and 691 downregulated genes, while after short-distance runs, only 13 up- and 8 downregulated genes (FC > |1.5|; p < 0.05) were found. These differentially expressed genes (DEGs) are involved in processes and pathways that are primarily related to stress response (interleukin production, activation of inflammatory system) but also to metabolism (carbohydrate catabolic process, lipid biosynthesis, NADP metabolic process). The most important genes involved in these processes therefore represent good candidates for the monitoring and evaluation of the performance of horses in order to avoid excessive demands when endurance performance is required, like ACOD1, CCL5, CD40LG, FOS, IL1R2, IL20RA, and IL22RA2, on the one hand, and, on the other hand, for assessing the suitability of a horse for endurance races, like GATA2, GYG1, HIF1A, MOGAT1, PFKFB3, PLIN5, SIK1, and STBD1.
Funder
Volkswagen Stiftung, Germany
Subject
Genetics (clinical),Genetics
Reference90 articles.
1. A missense mutation in the endothelin-B receptor gene is associated with Lethal White Foal Syndrome: An equine version of Hirschsprung disease;Metallinos;Mamm. Genome,1998 2. A missense mutation in the gene for melanocyte-stimulating hormone receptor (MC1R) is associated with the chestnut coat color in horses;Marklund;Mamm. Genome,1996 3. A missense variant in the coil1A domain of the keratin 25 gene is associated with the dominant curly hair coat trait (Crd) in horse;Morgenthaler;Genet. Sel. Evol.,2017 4. Littiere, T.O., Castro, G.H.F., Rodriguez, M., Bonafe, C.M., Magalhaes, A.F.B., Faleiros, R.R., Vieira, J.I.G., Santos, C.G., and Verardo, L.L. (2020). Identification and Functional Annotation of Genes Related to Horses’ Performance: From GWAS to Post-GWAS. Animals, 10. 5. Petersen, J.L., Mickelson, J.R., Rendahl, A.K., Valberg, S.J., Andersson, L.S., Axelsson, J., Bailey, E., Bannasch, D., Binns, M.M., and Borges, A.S. (2013). Genome-wide analysis reveals selection for important traits in domestic horse breeds. PLoS Genet., 9.
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