Comprehensive Analysis of miRNA and mRNA Expression Profiles during Muscle Development of the Longissimus Dorsi Muscle in Gannan Yaks and Jeryaks
Author:
Guo Dashan1, Wei Yali1, Li Xupeng1, Bai Yanbin1, Liu Zhanxin1, Li Jingsheng1, Chen Zongchang1, Shi Bingang1, Zhang Xiaolan1, Zhao Zhidong1ORCID, Hu Jiang1, Han Xiangmin1, Wang Jiqing1ORCID, Liu Xiu1, Li Shaobin1ORCID, Zhao Fangfang1
Affiliation:
1. Gansu Key Laboratory of Herbivorous Animal Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
Abstract
A hybrid offspring of Gannan yak and Jersey cattle, the Jeryak exhibits apparent hybrid advantages over the Gannan yak in terms of production performance and other factors. The small non-coding RNAs known as miRNAs post-transcriptionally exert a significant regulatory influence on gene expression. However, the regulatory mechanism of miRNA associated with muscle development in Jeryak remains elusive. To elucidate the regulatory role of miRNAs in orchestrating skeletal muscle development in Jeryak, we selected longissimus dorsi muscle tissues from Gannan yak and Jeryak for transcriptome sequencing analysis. A total of 230 (DE) miRNAs were identified in the longissimus dorsi muscle of Gannan yak and Jeryak. The functional enrichment analysis revealed a significant enrichment of target genes from differentially expressed (DE)miRNAs in signaling pathways associated with muscle growth, such as the Ras signaling pathway and the MAPK signaling pathway. The network of interactions between miRNA and mRNA suggest that some (DE)miRNAs, including miR-2478-z, miR-339-x, novel-m0036-3p, and novel-m0037-3p, played a pivotal role in facilitating muscle development. These findings help us to deepen our understanding of the hybrid dominance of Jeryaks and provide a theoretical basis for further research on the regulatory mechanisms of miRNAs associated with Jeryak muscle growth and development.
Funder
National Natural Science Foundation Discipline Team Project of Gansu Agricultural University Development and demonstration of high-efficiency production technology of yaks and cattle farming and animal husbandry cycle in pastoral areas of Qilian Mountains
Subject
Genetics (clinical),Genetics
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