Unveiling the Genetic Basis Underlying Rice Anther Culturability via Segregation Distortion Analysis in Doubled Haploid Population
Author:
Sun Bin12ORCID, Ding Xiaorui3, Ye Junhua1, Dai Yuting1, Cheng Can1, Zhou Jihua1, Niu Fuan1, Tu Rongjian1, Hu Qiyan4, Xie Kaizhen5, Qiu Yue3, Li Hongyu4, Feng Zhizun6, Shao Chenbing5, Cao Liming1, Zhang Anpeng2, Chu Huangwei1ORCID
Affiliation:
1. Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China 2. Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China 3. Development Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China 4. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China 5. MOE Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China 6. College of Agronomy, Shanxi Agricultural University, Jinzhong 030801, China
Abstract
Anther culture (AC) is a valuable technique in rice breeding. However, the genetic mechanisms underlying anther culturability remain elusive, which has hindered its widespread adoption in rice breeding programs. During AC, microspores carrying favorable alleles for AC are selectively regenerated, leading to segregation distortion (SD) of chromosomal regions linked to these alleles in the doubled haploid (DH) population. Using the AC method, a DH population was generated from the japonica hybrid rice Shenyou 26. A genetic map consisting of 470 SNPs was constructed using this DH population, and SD analysis was performed at both the single- and two-locus levels to dissect the genetic basis underlying anther culturability. Five segregation distortion loci (SDLs) potentially linked to anther culturability were identified. Among these, SDL5 exhibited an overrepresentation of alleles from the female parent, while SDL1.1, SDL1.2, SDL2, and SDL7 displayed an overrepresentation of alleles from the male parent. Furthermore, six pairs of epistatic interactions (EPIs) that influenced two-locus SDs in the DH population were discovered. A cluster of genetic loci, associated with EPI-1, EPI-3, EPI-4, and EPI-5, overlapped with SDL1.1, indicating that the SDL1.1 locus may play a role in regulating anther culturability via both additive and epistatic mechanisms. These findings provide valuable insights into the genetic control of anther culturability in rice and lay the foundation for future research focused on identifying the causal genes associated with anther culturability.
Funder
Shanghai Science and Technology Innovation Action Plan Project Shanghai Key Laboratory of Agricultural Genetics and Breeding
Subject
Genetics (clinical),Genetics
Reference50 articles.
1. A Mediterranean japonica rice (Oryza sativa) cultivar improvement through anther culture;Serrat;Euphytica,2013 2. Lantos, C., Jancso, M., Szekely, A., Szaloki, T., Venkatanagappa, S., and Pauk, J. (2023). Development of In Vitro Anther Culture for Doubled Haploid Plant Production in Indica Rice (Oryza sativa L.) Genotypes. Plants, 12. 3. Tripathy, S.K., Lenka, D., Prusti, A.M., Mishra, D., Swain, D., and Behera, S. (2019). Anther culture in rice: Progress and breeding perspective. Appl. Biol. Res., 21. 4. Lantos, C., Jancso, M., Szekely, A., Nagy, E., Szaloki, T., and Pauk, J. (2022). Improvement of Anther Culture to integrate Doubled Haploid Technology in Temperate Rice (Oryza sativa L.) Breeding. Plants, 11. 5. Gametic embryogenesis and haploid technology as valuable support to plant breeding;Plant Cell Rep.,2011
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