Abstract
Dicyclopropanated 5-vinyl-2-norbornene (dcpVNB) is a strained polycyclic hydrocarbon compound with a high energy content, which makes it promising for the development of propellant components based on it. In this work, the genotoxic properties of dcpVNB were studied using whole-cell lux-biosensors based on Escherichia coli and Bacillus subtilis. It was shown that the addition of dcpVNB to bacterial cells leads to the appearance of DNA damage inducing the SOS response and Dps expression with slight activation of the OxyR-mediated response to oxidative stress. The highest toxic effect of dcpVNB is detected by the following lux-biosensors: E. coli pColD-lux, E. coli pDps, B. subtilis pNK-DinC, and B. subtilis pNK-MrgA, in which the genes of bacterial luciferases are transcriptionally fused to the corresponding promoters: Pcda, Pdps, PdinC, and PmrgA. It was shown that lux-biosensors based on B. subtilis, and E. coli are almost equally sensitive to dcpVNB, which indicates the same permeability to this compound of cell wall of Gram-positive and Gram-negative bacteria. The activation of Pdps after dcpVNB addition maintains even in oxyR mutant E. coli strains, which means that the Pdps induction is only partially determined by the OxyR/S regulon. Comparison of specific stress effects caused by dcpVNB and 2-ethyl(bicyclo[2.2.1]heptane) (EBH), characterized by the absence of cyclopropanated groups, shows that structural changes in hydrocarbons could significantly change the mode of toxicity.
Funder
Russian Science Foundation
Ministry of Science and Higher Education of the Russian Federation
Subject
Clinical Biochemistry,General Medicine,Analytical Chemistry,Biotechnology,Instrumentation,Biomedical Engineering,Engineering (miscellaneous)
Reference38 articles.
1. Detailed Kinetic Study of the Ring Opening of Cycloalkanes by CBS-QB3 Calculations;Sirjean;J. Phys. Chem. A,2006
2. Occupational Hazards of Missile Operations with Special Regard to the Hydrazine Propellants;Back;Aviat Space Environ. Med.,1978
3. Unsymmetrical Dimethylhydrazine and Related Compounds in the Environment: Recent Updates on Pretreatment, Analysis, and Removal Techniques;Hu;J. Hazard Mater.,2022
4. Actual Directions in Study of Ecological Consequences of a Highly Toxic 1,1-Dimethylhydrazine-Based Rocket Fuel Spills;Kenessov;Chem. Bull. Kazakh Natl. Univ.,2012
5. GC-MS Determination of 1-Methyl-1H-1,2,4-Triazole in Soils Affected by Rocket Fuel Spills in Central Kazakhstan;Kenessov;Chromatographia,2008
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