Affiliation:
1. Department of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, Gyeongsangbuk-do, Republic of Korea
2. Research Institute for Innovative Animal Science, Kyungpook National University, Sangju 37224, Gyeongsangbuk-do, Republic of Korea
Abstract
A synthetic pyrethroid pesticide, bifenthrin, has been commonly used as an effective exterminator, although the rise in its usage has raised concerns regarding its effects on the environment and public health, including reproduction, globally. The current study investigated the function-related molecular disparities and mechanisms in bifenthrin-exposed sperm cells and the underlying mechanism. Therefore, epididymal spermatozoa were released, and various concentrations of bifenthrin were treated (0.1, 1, 10, and 100 μM) to evaluate their effects on sperm. The findings showed that although bifenthrin had no effect on sperm viability, various other sperm functions (e.g., motility, spontaneous acrosome reaction, and capacitation) related to male fertility were decreased, commencing at a 1 µM treatment. Molecular studies revealed nine differentially expressed sperm proteins that were implicated in motile cilium assembly, sperm structure, and metabolic processes. Furthermore, bifenthrin affected sperm functions through abnormal diminution of the expression of specific sperm proteins. Collectively, these findings provide greater insights into how bifenthrin affects male fertility at the molecular level.
Funder
Korean government
Ministry of Education (MOE) of Korea
National Research Foundation (NRF) of Korea
Subject
Chemical Health and Safety,Health, Toxicology and Mutagenesis,Toxicology
Reference54 articles.
1. Golden Age of Insecticide Research: Past, Present, or Future?;Casida;Annu. Rev. Entomol.,1998
2. Stabilization of sodium channel states by deltamethrin in mouse neuroblastoma cells;Chinn;J. Physiol.,1986
3. Kinetic properties of single sodium channels modified by fenvalerate in mouse neuroblastoma cells;Holloway;Pflügers Arch. Eur. J. Physiol.,1989
4. Krieger, R.I., and Krieger, W.C. (2001). Handbook of Pesticide Toxicology, Academic Press. [2nd ed.].
5. Neurotoxic actions of pyrethroid insecticides;Soderlund;Annu. Rev. Entomol.,1989