Untargeted LC-QTOF-MS Analysis of Metabolites Produced by Penicillium brevicompactum during the Bioconversion of Ganoderic Acid A

Abstract

The repurposing of expired drugs through bioconversion remains one of the most crucial research milestones, as this practice reduces drug contamination while producing compounds of significance. The present study investigated the bioconversion of ganoderic acid A (GAA) using Penicillium brevicompactum over a period of 3, 6 and 9 days. The GAA intensity reduced from 22,099 cps on day 0 to 11,040, 4700 and 18,126 cps on day 3, 6 and 9, respectively, thus demonstrating the degradation of GAA over time. The produced metabolites that were recovered using ethyl acetate as a solvent were determined using LC-QTOF-MS. P. brevicompactum produced a variety of compounds in the absence of GAA, while in its presence, it was observed that P. brevicompactum was able to convert GAA and produced ganomastenol A/B/D, vitamin E succinate, and aminopregnane on day 3, while on day 6, armillaripin and ganolucidic acid A were produced. After 9 days of operation, vitamin E succinate, ganolucidic acid A and lucilactaene were produced. The present study is the first report on the ability of P. brevicompactum to bioconvert GAA. The identified metabolites have been established to possess bioactivity against various ailments, thus contributing to the discovery of new drugs.