Trapa bispinosa Roxb. Pericarp Extract Exerts 5α-Reductase Inhibitory Activity in Castrated Benign Prostatic Hyperplasia Model Mice
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Published:2023-07-21
Issue:14
Volume:24
Page:11765
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Fujita Takashi1ORCID, Aoyama Tomoko1, Uemura Tomohiro2, Takeshita Shouko2, Yamasaki Takuto2, Heijou Hiroko2, Morimoto Koji1
Affiliation:
1. Department of Pharmaceutical Sciences, Ritsumeikan University, Kusatsu 525-8577, Japan 2. Hayashikane Sangyo Co., Ltd., Yamaguchi 750-8608, Japan
Abstract
Trapa bispinosa Roxb. pericarp extract (TBE) has a polyphenol-rich composition and exhibits potent antioxidant and anti-glycation activities in vitro. In the present study, we investigated the inhibitory effects of TBE on 5α-reductase in vitro using LNCaP cells and in vivo using a mouse model of castrated benign prostatic hyperplasia. TBE showed concentration-dependent inhibitory effects in the 5α-reductase (5αR) activity assay. In a reporter assay using AR-Luc/LNCaP cells, TBE inhibited the activity induced by testosterone, but not that induced by dihydrotestosterone. TBE also suppressed prostate cell proliferation, prostate-specific antigens, and transmembrane protease serine 2 expression in a castrated benign prostatic hyperplasia mouse model. In addition, ellagic acid, but not gallic acid, decreased 5αR and AR-Luc activities. Together, these results suggest a potential role for TBE in benign prostatic hyperplasia through inhibition of 5αR.
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference31 articles.
1. Iwaoka, Y., Suzuki, S., Kato, N., Hayakawa, C., Kawabe, S., Ganeko, N., Uemura, T., and Ito, H. (2021). Characterization and Identification of Bioactive Polyphenols in the Trapa bispinosa Roxb. Pericarp Extract. Molecules, 26. 2. Phytochemical and antioxidant analysis of medicinal and food plants towards bioactive food and pharmaceutical resources;Yu;Sci. Rep.,2021 3. Skin-related enzyme inhibitory activity by hydrolyzable polyphenols in water chestnut (Trapa natans) husk;Yasuda;Biosci. Biotechnol. Biochem.,2021 4. Trapa bispinosa Roxb.: A Review on Nutritional and Pharmacological Aspects;Adkar;Adv. Pharmacol. Sci.,2014 5. Youness, R.A., Kamel, R., Elkasabgy, N.A., Shao, P., and Farag, M.A. (2021). Recent Advances in Tannic Acid (Gallotannin) Anticancer Activities and Drug Delivery Systems for Efficacy Improvement; A Comprehensive Review. Molecules, 26.
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