Exploring the Genetic Diversity of Epstein–Barr Virus among Patients with Gastric Cancer in Southern Chile-Reference-Cited by-同舟云学术

Exploring the Genetic Diversity of Epstein–Barr Virus among Patients with Gastric Cancer in Southern Chile

Published:2023-07-10 Issue:14 Volume:24 Page:11276
ISSN:1422-0067
Container-title:International Journal of Molecular Sciences
language:en
Short-container-title:IJMS

Author:

Reyes María Elena¹,Zanella Louise²³^ORCID,Riquelme Ismael¹^ORCID,Buchegger Kurt⁴⁵,Mora-Lagos Bárbara¹,Guzmán Pablo⁶,García Patricia⁷^ORCID,Roa Juan C.⁷^ORCID,Ili Carmen Gloria⁴^ORCID,Brebi Priscilla⁴

Affiliation:

1. Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Temuco 4810101, Chile

2. Doctorado en Ciencias Médicas, Universidad de La Frontera, Temuco 4811230, Chile

3. Núcleo Milenio de Sociomedicina, Santiago 7560908, Chile

4. Laboratory of Integrative Biology (LIBi), Millennium Institute on Immunology and Immunotherapy, Center of Excellence in Translational Medicine-Scientific and Technological Bioresource Nucleus-(-CEMT-BIOREN), Universidad de La Frontera, Temuco 4810296, Chile

5. Departamento de Ciencias Básicas, Facultad de Medicina, Universidad de La Frontera, Temuco 4811322, Chile

6. Pathology Department, School of Medicine, Universidad de La Frontera, Temuco 4781176, Chile

7. Millennium Institute on Immunology and Immunotherapy, Department of Pathology, School of Medicine, Pontificia Universidad Católica de Chile, Santiago 8330024, Chile

Abstract

The Epstein–Barr virus (EBV) has been associated with gastric cancer (GC), one of the deadliest malignancies in Chile and the world. Little is known about Chilean EBV strains. This study aims to investigate the frequency and genetic diversity of EBV in GC in patients in southern Chile. To evaluate the prevalence of EBV in GC patients from the Chilean population, we studied 54 GC samples using the gold standard detection method of EBV-encoded small RNA (EBER). The EBV-positive samples were subjected to amplification and sequencing of the Epstein–Barr virus nuclear protein 3A (EBNA3A) gene to evaluate the genetic diversity of EBV strains circulating in southern Chile. In total, 22.2% of the GC samples were EBV-positive and significantly associated with diffuse-type histology (p = 0.003). Phylogenetic analyses identified EBV-1 and EBV-2 in the GC samples, showing genetic diversity among Chilean isolates. This work provides important information for an epidemiological follow-up of the different EBV subtypes that may cause GC in southern Chile.

Funder

National Commission for Scientific and Technological Research

National Funding for Scientific and Technologic Development of Chile

Advanced Research Grant from Research Direction from Universidad Autónoma de Chile

Research Office of Universidad de La Frontera

FONDEF Idea

Millennium Institute on Immunology and Immunotherapy

ANID–MILENIO

European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Program

ANID/FONDAP CECAN

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

Link

https://www.mdpi.com/1422-0067/24/14/11276/pdf

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