Study on the Characteristics and Mechanism of Inorganic Nitrogen Nutrients Preferential Assimilation by Phaeocystis globosa

Author:

Guo Honghong123ORCID,Wang Wentao234,Han Jishu1,Zhu Jianan23ORCID,Wang Zhongshi234ORCID,He Liyan23,Song Xiuxian234,Yu Zhiming234

Affiliation:

1. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China

2. CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

3. Laboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao 266237, China

4. University of Chinese Academy of Sciences, Beijing 100049, China

Abstract

Phaeocystis globosa is an important harmful algal species that is globally distributed. Previous studies have indicated that P. globosa preferentially uptake nitrate, but the underlying mechanism is still unclear. To further verify this preference and reveal the underlying mechanism, the assimilation rates of nitrate and ammonium by P. globosa at different concentrations was quantitatively studied by using a nitrogen stable isotope (15N) tracer technique, and the regulatory mechanism was determined from the physiological and biochemical responses. The findings revealed that the preferential assimilation of nitrate by P. globosa was influenced by the ambient ammonium concentration. When the ambient concentration of ammonium was less than approximately 3.5 μmol·L−1, the assimilation rates of nitrate form P. globosa were as high as 1.05 × 10−5 μmol·L−1·h−1·cell−1. Even though the nitrate assimilation in P. globosa was inhibited at ammonium concentrations greater than this threshold, nitrate assimilation could not be completely suppressed. The activity of NR and the expression of related genes in P. globosa were also affected by ammonium. In addition, 15N signals originally labeled nitrate accumulated in ammonium. This indicated that P. globosa was likely to reduce excess nitrate to ammonium and subsequently release it into the substrate, which might be an important energy dissipation mechanism for P. globosa. These results expand the classical understanding of the utilization of nitrogen nutrients by marine phytoplankton, and offer valuable resources for comprehending the mechanism of harmful algal blooms.

Funder

Shandong Provincial Natural Science Foundation Committee

Intergovernmental Innovation Cooperation Project of the Ministry of Science and Technology

Laoshan Laboratory

National Natural Science Foundation of China

Publisher

MDPI AG

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