Preventive Effects of Chlorogenic Acid on Alveolar Bone Loss in Ligature-Induced Periodontitis in Mice

Author:

Nishida Yuka1,Shimada Katsumitsu2,Horibe Kanji3ORCID,Seki Kousuke4,Murai Yoshinori5,Sogawa Chiharu6ORCID,Murakami Satoshi2,Nakamura Hiroaki3,Masuda Yuji7,Sogawa Norio1

Affiliation:

1. Department of Molecular Engineering and Drug Developmental Sciences, Graduate School of Oral Medicine, Matsumoto Dental University, 1780 Hirooka Gobara, Shiojiri 399-0781, Japan

2. Department of Clinical Pathophysiology, Matsumoto Dental University, 1780 Hirooka Gobara, Shiojiri 399-0781, Japan

3. Department of Oral Histology, Matsumoto Dental University, 1780 Hirooka Gobara, Shiojiri 399-0781, Japan

4. Nagano Vegetable and Ornamental Crops Experiment Station, 1066-1 Tokoo, Souga, Shiojiri 399-6461, Japan

5. Department of Botany, National Museum of Nature and Science, 4-1-1 Amakubo, Tsukuba 305-0005, Japan

6. Department of Clinical Engineering, Faculty of Life Sciences, Hiroshima Institute of Technology, 2-1-1 Miyake, Saeki-ku, Hiroshima 731-5193, Japan

7. Division of Maxillofacial Biology, Institute for Oral Science, Matsumoto Dental University, 1780 Hirooka Gobara, Shiojiri 399-0781, Japan

Abstract

Chlorogenic acid (CGA) is a polyphenol that is present in coffee beans, many vegetables, and fruits. Since CGA has been reported to exert antioxidant and anti-inflammatory effects, it is expected to protect against periodontitis. In the present study, we used a ligature-induced experimental periodontitis model and investigated the beneficial effects of CGA against alveolar bone resorption caused by experimental periodontitis. To examine the inhibitory effects of CGA on bone loss, a ligature was wrapped around the maxillary right second molar, and CGA was intraperitoneally injected once a day for 2 weeks. In another experiment to investigate the restorative effects of CGA on bone loss, a ligature was wrapped around the maxillary right second molar for 2 weeks, it was then removed, and CGA was intraperitoneally injected once a day for 2 weeks. At the end of the experiments, the maxillae were removed, and CT images were taken. Alveolar bone loss was measured as the distance from the cement–enamel junction to the alveolar crest. The statistical analysis was performed using GraphPad Prism6 (Dunn’s multiple comparison test). The results revealed that the ratio of the buccal alveolar bone loss (vs. the bone loss on the nonligated side) induced by ligation was significantly decreased by the administration of CGA (5 mg/kg) for 2 weeks. Moreover, the bone loss ratio on the buccal and palatal sides after 2 weeks of ligation was significantly decreased by the 2-week administration of CGA (5 mg/kg). The present results revealed that CGA exerted preventive effects against alveolar bone loss caused by experimental periodontitis.

Funder

Nagano Society for the Promotion of Science

Publisher

MDPI AG

Subject

Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science

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