Low-Cost, Compact, and Rapid Bio-Impedance Spectrometer with Real-Time Bode and Nyquist Plots

Abstract

Bioelectric impedance spectroscopy (BIS) has been widely used to study the electrical properties of biological tissue based on the characteristics of the complex electrical impedance dispersions. One of the problems in using the BIS method is the length of time required for the data acquisition process and possibly data analysis as well. In this research, a compact and work rapidly BIS instrumentation system has been developed at a low cost. It is designed to work in the frequency range of 100 Hz to 100 kHz, which is generally used in the fields of biophysics and medical physics. The BIS instrumentation system is built using several integrated modules. The modules are an AC current source to produce a selectable injection current; a data acquisition system to measure voltage, current, and phase difference rapidly and simultaneously; and software to calculate and display measurement results in the form of Bode and Nyquist plots in real time. The developed BIS system has been validated using a simple RC circuit as the sample being tested. The average time needed in the process of data acquisition and analysis until the formation of impedance dispersion curves in the form of Bode and Nyquist plots, for 54 sample frequencies, is less than one minute. The system is able to identify R and C values of the sample with a maximum error of 1.5%. In addition, some simple application examples are also presented in this paper.