Diagnosis of a Rabbit Hemorrhagic Disease Virus 2 (RHDV2) and the Humoral Immune Protection Effect of VP60 Vaccine
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Published:2023-08-08
Issue:8
Volume:45
Page:6605-6617
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ISSN:1467-3045
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Container-title:Current Issues in Molecular Biology
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language:en
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Short-container-title:CIMB
Author:
Li Zhaoming1ORCID, Song Kaimin1ORCID, Du Yongzhen1, Zhang Zhuanglong2, Fan Rupeng1, Zheng Pimiao2ORCID, Liu Jianzhu1ORCID
Affiliation:
1. College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, China 2. Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai’an 271018, China
Abstract
Rabbit hemorrhagic disease (RHD) is known as rabbit plague and hemorrhagic pneumonia. It is an acute, septic, and highly fatal infectious disease caused by the Lagovirus rabbit hemorrhagic disease virus (RHDV) in the family Caliciviridae that infects wild and domestic rabbits and hares (lagomorphs). At present, RHDV2 has caused huge economic losses to the commercial rabbit trade and led to a decline in the number of wild lagomorphs worldwide. We performed a necropsy and pathological observations on five dead rabbits on a rabbit farm in Tai’an, China. The results were highly similar to the clinical and pathological changes of typical RHD. RHDV2 strain was isolated and identified by RT-PCR, and partial gene sequencing and genetic evolution analysis were carried out. There were significant differences in genetic characteristics and antigenicity between RHDV2 and classical RHDV strain, and the vaccine prepared with the RHDV strain cannot effectively prevent rabbit infection with RHDV2. Therefore, we evaluated the protective efficacy of a novel rabbit hemorrhagic virus baculovirus vector inactivated vaccine (VP60) in clinical application by animal regression experiment. The result showed that VP60 could effectively induce humoral immunity in rabbits. The vaccine itself had no significant effect on the health status of rabbits. This study suggested that the clinical application of VP60 may provide new ideas for preventing the spread of RHD2.
Funder
Xinjiang Key Laboratory of Animal Infectious Diseases
Subject
Microbiology (medical),Molecular Biology,General Medicine,Microbiology
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