Effects of Acid Rain Stress on the Physiological and Biochemical Characteristics of Three Plant Species

Abstract

The physiological and biochemical indicators of plants reflect the plant’s adaptation to environmental changes and provide information for the planting and management of acid-resistant tree species. To analyze the responses of typical tree species to recent changes in acid rain conditions in Jinyun Mountain, Chongqing, we focused on three representative tree species in the Jinyun Mountain area of Chongqing: Pinus massoniana, Phyllostachys edulis, and Cinnamomum camphora. A mixed acid rain experiment with five gradients of natural rainfall (NR) and pH values of 7.0, 4.5, 3.5, and 2.5 was conducted in May 2021. The changes in physiological and biochemical indicators (net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate, light saturation point, light compensation point, apparent quantum efficiency, dark respiration rate, soluble sugar, starch, soluble protein, proline, malondialdehyde, and antioxidant enzyme activity) were determined. The results show the following: 1. Compared with other treatments, NR and slightly acidic rain increased the relative chlorophyll content in plant seedlings. 2. The synthesis of soluble sugars, starches, and soluble proteins was inhibited to different degrees in the three species under acid rain stress at pH ≤ 3.5. 3. The enzyme activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) initially increased and then decreased with an increase in acidity. 4. Acid rain treatments with pH ≤ 4.5 reduced the net photosynthetic rate (Pn) of plants; the higher the acidity, the lower the Pn. Conclusion: A comprehensive comparison of the indicators revealed that NR and mild acid rain enhanced the plant seedlings’ physiological and biochemical characteristics. A pH of 3.5 was the threshold where acid rain had an adverse effect on Pinus massoniana, Phyllostachys edulis, and Cinnamomum camphora. The high indicator values for NR indicate that these tree species have adapted to current conditions in the Jinyun Mountain area of Chongqing. This study provides new information for selecting tree species adapted to the acid rain environment in Jinyun Mountain, Chongqing.