Design and Study of a Reflector-Separated Light Dispersion-Compensated 3D Microscopy System

Author:

Li Hui12,Tan Xin1,Jiao Qingbin1,Li Yuhang12,Liu Siqi12,Pei Jian12,Zhang Jiahang12,Zhang Wei12,Xu Liang1

Affiliation:

1. Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Beijing 130033, China

2. University of Chinese Academy of Sciences, Beijing 100049, China

Abstract

The secondary-phase grating-based tomographic microscopy system, which is widely used in the biological and life sciences, can observe all the sample multilayer image information simultaneously because it has multifocal points. However, chromatic aberration exists in the grating diffraction, which seriously affects the observation of the image. To correct the chromatic aberration of the tomographic microscope system, this paper proposes a system that adopts blazed gratings and angle-variable reflectors as chromatic aberration correction devices according to the principle of dispersion compensation and Fourier phase-shift theory. A reflector-separated light dispersion-compensated 3D microscopy system is presented to achieve chromatic aberration correction while solving the problem of multilayer image overlap. The theoretical verification and optical design of the system were completed using ZEMAX software. The results show that the proposed system reduced the chromatic aberration of ordinary tomographic microscopy systems by more than 90%, retaining more wavelengths of light information. In addition, the system had a relatively wide range in the color difference compensation element installation position, reducing the difficulty of dispersion compensation element installation. Overall, the results indicate that the proposed system is effective in reducing chromatic aberration in grating diffraction.

Funder

National Natural Science Foundation of China

Capital construction funds in Jilin Province in 2023

Changchun science and technology development plan project

Jilin Province Science and Technology Development Plan Project

Publisher

MDPI AG

Subject

Electrical and Electronic Engineering,Biochemistry,Instrumentation,Atomic and Molecular Physics, and Optics,Analytical Chemistry

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