Discovery and Characterization of MaK: A Novel Knottin Antimicrobial Peptide from Monochamus alternatus

Author:

Han Xiaohong12,Zhou Tong3,Hu Xinran12,Zhu Yukun12,Shi Zengzeng12,Chen Shi12,Liu Yunfei12,Weng Xiaoqian12,Zhang Feiping12,Wu Songqing14ORCID

Affiliation:

1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China

2. Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Province University, Fujian Agriculture and Forestry University, Fuzhou 350002, China

3. Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

4. Fujian Colleges and Universities Engineering Research Institute of Conservation and Utilization of Natural Bioresources, Fujian Agriculture and Forestry, Fuzhou 350002, China

Abstract

Knottin-type antimicrobial peptides possess exceptional attributes, such as high efficacy, low vulnerability to drug resistance, minimal toxicity, and precise targeting of drug sites. These peptides play a crucial role in the innate immunity of insects, offering protection against bacteria, fungi, and parasites. Knottins have garnered considerable interest as promising contenders for drug development due to their ability to bridge the gap between small molecules and protein-based biopharmaceuticals, effectively addressing the therapeutic limitations of both modalities. This work presents the isolation and identification of a novel antimicrobial peptide derived from Monochamus alternatus. The cDNA encodes a 56-amino acid knottin propeptide, while the mature peptide comprises only 34 amino acids. We have labeled this knottin peptide as MaK. Using chemically synthesized MaK, we evaluated its hemolytic activity, thermal stability, antibacterial properties, and efficacy against nematodes. The results of this study indicate that MaK is an exceptionally effective knottin-type peptide. It demonstrates low toxicity, superior stability, potent antibacterial activity, and the ability to suppress pine wood nematodes. Consequently, these findings suggest that MaK has potential use in developing innovative therapeutic agents to prevent and manage pine wilt disease.

Funder

National Natural Science Foundation of China

National Key R & D Program of China

Forestry Key Program of Science and Technology in Fujian Province

Natural Science Foundation of Fujian Province, China

Forestry Programs of Science and Technology in Fujian Province

Science and Technology Program of Fujian Province

Forestry Science Research Project of Fujian Forestry Department

Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University

Undergraduate Training Program for Innovation and Entrepreneurship of China

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Potential and Limitation of Peptides from Native Plants of Uttarakhand;International Journal of Peptide Research and Therapeutics;2024-09-03

2. The Neurotranscriptome of Monochamus alternatus;International Journal of Molecular Sciences;2024-04-22

3. Natural Compounds for Inhibiting Oncogenic Microbiota: A Review of Their Therapeutic Applications;Pathogens Associated with the Development of Cancer in Humans;2024

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