Isolation and Maximisation of Extraction of Mangiferin from the Root of Salacia chinensis L.

Abstract

Mangiferin has been reported to exhibit anti-viral, anti-cancer, anti-diabetic, immunomodulatory and hepatoprotective properties. This study aimed to develop an HPLC method to isolate mangiferin from Salacia chinensis L. root; investigate the impact of solvents on yield; optimise the ultrasound-assisted extraction (UAE) technique; and compare mangiferin yield with continuously shaking extraction (CSE) and decoction techniques. The results showed that mangiferin, with a purity of over 88%, could be achieved by HPLC using a mixture of solvent A (water: acetonitrile: orthophosphoric acid, 96.8:3:0.2 (v/v/v)) and solvent B (acetonitrile). Solvent type significantly affected the extraction yield of mangiferin, and a mixture of acetone and water gave the highest extraction yield, as compared to other solvents or mixtures. UAE conditions, such as ultrasonic power, temperature, time and concentration of acetone significantly affected the extraction of mangiferin. Optimal UAE conditions were at an ultrasonic power of 250 W, temperature of 50 °C, acetone concentration of 40% and extraction time of 60 min. These optimal conditions could extract approximately 92 mg, whereas CSE and decoction only extracted 89.20 mg and 58.71 mg of mangiferin, respectively, from 1 g of S. chinensis root. Therefore, these UAE conditions are recommended for the extraction of mangiferin from S. chinensis root for further utilisation.