Automated Image Analysis for Retention Determination in Centrifugal Partition Chromatography

Abstract

In Centrifugal Partition Chromatography, two immiscible liquids are used as mobile and stationary phases. During operation, bleeding of the stationary phase cannot be eliminated completely. For optimal separation performance, however, it is crucial to maintain sufficient amounts of stationary phase in the system, which is quantitatively measured by the retention value. With an online measurement of that retention value, it is possible to make predictions about the separation performance of the system. Therefore, an image processing algorithm was developed in this study, allowing quick and effortless online evaluation of retention by image analysis. Finally, the results were compared with proven analysis methods to evaluate the measurements’ validity. With the help of the new algorithm, it was possible to improve the number of pictures analyzed per time and the precision compared to the previously used technique.