Affiliation:
1. Institute of Advance Carbon Conversion Technology, Huaqiao University, Xiamen 361021, China
2. College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
3. Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China
4. Engineering Research Center of Forest Bio-Preparation, Northeast Forestry University, Harbin 150040, China
Abstract
Flavonoids exhibit many biological properties, so it is very important to find an efficient and green method to extract them from plant materials. In this paper, DES-MAE (deep eutectic solvent-based microwave-assisted extraction) technique was developed to extract the seven major active flavonoids from Ribes mandshuricum leaves, namely, trifolin, isoquercetin, rutin, astragalin, quercetin, hyperoside, and kaempferol. After the completion of the extraction process, macroporous adsorption resin was used for the purification of seven flavonoids. The BBD (Box–Behnken design) method combined with RSM (response surface methodology) was applied to acquire the optimal operating conditions of DES-MAE. The optimal parameters were: temperature: 54 °C, time: 10 min, extraction solvent: choline chloride/lactic acid with a 1:2 mass ratio, water content: 25%, and liquid/solid ratio: 27 mL/g. The yields of the seven target flavonoids were 4.78, 2.57, 1.25, 1.15, 0.34, 0.32, and 0.093 mg/g DW (dry weight), respectively. The direct purification of trifolin, isoquercetin, rutin, astragalin, quercetin, hyperoside, and kaempferol in DES-MAE solution was achieved by using macroporous resin X-5. The recoveries were 87.02%, 81.37%, 79.64%, 87.13%, 97.36%, 88.08%, and 99.39%, respectively. The results showed that DES-MAE followed by MRCC (macroporous resin column chromatography) represents a promising approach to extracting and separating active components from plants.
Funder
National Key R&D Program of China
Scientific Research Funds of Huaqiao University
Subject
Filtration and Separation,Analytical Chemistry