Circular Hazelnut Protection by Lignocellulosic Waste Valorization for Nanopesticides Development

Abstract

Hazelnut represents a relevant agro-food supply chain in many countries worldwide. Several biological adversities threaten hazelnut cultivation, but among them bacterial blight is one of the most feared and pernicious since its control can be achieved only by prevention through the observation of good agricultural practices and the use of cupric salts. The aim of this work was to evaluate the lignocellulosic biomasses obtained from hazelnut pruning and shelling residues as a renewable source of cellulose nanocrystals and lignin nanoparticles and to investigate their antimicrobial properties against hazelnut bacterial blight. Cellulose nanocrystals were obtained through an acid hydrolysis after a chemical bleaching, while lignin nanoparticles were synthesized by a solvent–antisolvent method after an enzymatic digestion. Both collected nanomaterials were chemically and morphologically characterized before being tested for their in vitro and in vivo antibacterial activity and biocompatibility on hazelnut plants. Results indicated the selected biomasses as a promising starting material for lignocellulosic nanocarriers synthesis, confirming at the same time the potential of cellulose nanocrystals and lignin nanoparticles as innovative tools to control hazelnut bacterial blight infections without showing any detrimental effects on the biological development of treated hazelnut plants.