One Pot Synthesis of Copper Oxide Nanoparticles for Efficient Antibacterial Activity

Abstract

The unique semiconductor and optical properties of copper oxides have attracted researchers for decades. However, using fruit waste materials such as peels to synthesize the nanoparticles of copper oxide (CuO NPs) has been rarely described in literature reviews. The main purpose of this part of the research was to report on the CuO NPs with the help of apple peel extract under microwave irradiation. Metal salts and extracts were irradiated at 540 W for 5 min in a microwave in a 1:2 ratio. The crystallinity of the NPs was confirmed by the XRD patterns and the crystallite size of the NPs was found to be 41.6 nm. Elemental mapping of NPs showed homogeneous distributions of Cu and O. The NPs were found to contain Cu and O by EDX and XPS analysis. In a test involving two human pathogenic microbes, NPs showed antibacterial activity and the results revealed that the zone of inhibition grew significantly with respect to the concentration of CuO NPs. In a biofilm, more specifically, NPs at 25.0 µg/mL reduced mean thickness and biomass values of S. aureus and E. coli biofilms by >85.0 and 65.0%, respectively, with respect to untreated controls. In addition, environmentally benign materials offer a number of benefits for pharmaceuticals and other biomedical applications as they are eco-friendly and compatible.