A Thorough Insight into the Biofilm Properties of Potential Fish Pathogen Tenacibaculum discolor Strain FMCC B487

Author:

Schoina Eirini1ORCID,Marchand Laetitia2,Zykwinska Agata2ORCID,Sinquin Corinne2ORCID,Leroi Françoise2,Delbarre-Ladrat Christine2ORCID,Nychas George-John E.1ORCID

Affiliation:

1. Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, School of Food and Nutritional Sciences, Agricultural University of Athens, 11855 Athens, Greece

2. Ifremer, MASAE Microbiologie Aliment Santé Environment, F-44000 Nantes, France

Abstract

Tenacibaculum discolor develops biofilm in marine aquaculture production tanks and is identified as one of the causative agents of tenacibaculosis, a bacterial disease that causes significant losses in marine aquaculture production. In this study, the biofilm characteristics of T. discolor strain FMCC B487 were evaluated. Cell growth and biofilm formation and development were studied in miniaturized assays to assess the effect of different levels of environmental factors temperature and salinity, as well as the presence of monosaccharides potentially found in aquaculture hatcheries. The ability of the strain to grow and develop strong biofilms in ambient to high temperatures and at salinities above 20 g/L was shown. Mannose was the monosaccharide with the most prominent impact on the T. discolor strain FMCC B487 biofilm. The composition of planktonic cell extract, biofilm extracts, and extracellular polymeric substances (EPS) produced by T. discolor strain FMCC B487 were investigated by means of colorimetric and fluorometric assays as well as analyses by electrophoresis, gas chromatography, and high-performance size-exclusion chromatography coupled with a multiangle light scattering detector, revealing the dominance of proteins and lipids and the absence of high-molecular-weight polysaccharides. This information may serve as a basis for considering anti-biofilm strategies against the pathogen T. discolor.

Funder

European Union’s Horizon

Publisher

MDPI AG

Reference61 articles.

1. FAO (2022). In Brief to the State of World Fisheries and Aquaculture 2022. Towards Blue Transformation, FAO.

2. A review of the main bacterial fish diseases in mariculture systems;Toranzo;Aquaculture,2005

3. Microbial biofilms in seafood: A food-hygiene challenge;Mizan;Food Microbiol.,2015

4. Isolation, Characterization and Virulence Potential of Tenacibaculum dicentrarchi in Salmonid Cultures in Chile;Irgang;Transbound. Emerg. Dis.,2016

5. Identification and typing of fish pathogenic species of the genus Tenacibaculum;Santos;Appl. Microbiol. Biotechnol.,2018

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