Abstract
Lipopolysaccharide (LPS) can trigger a series of immune reactions, leading to the occurrence of disease and a decrease in the growth performance of geese. However, the mechanisms of LPS in geese muscle development have not been reported. This study aimed to investigate the effects and mechanisms of LPS on proliferation and differentiation of goose embryonic myoblasts. Embelin and belnacasan combined with LPS were used to explore these effects. Our results demonstrated that LPS significantly induced inflammatory cytokine production in both proliferation and differentiation stages. LPS and embelin treatment significantly improved the proliferation ability (p < 0.05), while LPS reduced the differentiation ability of goose embryonic myoblasts. By adding embelin, the differentiation ability of myoblasts was enhanced, while by adding belnacasan, LPS treatment led to a lower differentiation ability. Combined with the correlation of the expression levels of myogenic, cell cycle, and inflammatory-related genes and proteins, it is speculated that one of the reason for the decrease of differentiation ability of goose embryo myoblasts induced by LPS is the increase of the expression levels of pro-inflammatory factors. Moreover, LPS, embelin and belnacasan, and LPS treatments could significantly increase the apoptosis rate of goose embryonic myoblasts. Taken together, these findings suggest that LPS promotes the proliferation and differentiation of goose embryonic myoblasts by promoting cytokine expression and appropriate apoptosis processes. These findings lay a foundation for the study of the mechanisms of LPS in goose muscle development.
Funder
Key-Area Research and Development Program of Guangdong Province
Science and Technology Planning Project of Guangzhou
National College Students’ innovation and entrepreneurship training program
Reference43 articles.
1. Current situation of waterfowl industry in 2019, future development trend and suggestions;Chin. J. Anim. Sci.,2020
2. LPS induction of gene expression in human monocytes;Cell. Signal.,2001
3. Effects of florfenicol on early cytokine responses and survival in murine endotoxemia;Int. Immunopharmacol.,2008
4. LPS/TLR4 signal transduction pathway;Cytokine,2008
5. LPS, TLR4 and infectious disease diversity;Nat. Rev. Microbiol.,2005
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献