IbINV Positively Regulates Resistance to Black Rot Disease Caused by Ceratocystis fimbriata in Sweet Potato
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Published:2023-11-17
Issue:22
Volume:24
Page:16454
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Yang Dongjing1, Bian Xiaofeng2, Kim Ho Soo3ORCID, Jin Rong1, Gao Fangyuan1, Chen Jingwei1, Ma Jukui1, Tang Wei1, Zhang Chengling1, Sun Houjun1, Xie Yiping1, Li Zongyun4ORCID, Kwak Sang-Soo3ORCID, Ma Daifu1
Affiliation:
1. Key Laboratory of Biology and Genetic Improvement of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Xuzhou 221131, China 2. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China 3. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Daejeon 34141, Republic of Korea 4. College of Life Science, Jiangsu Normal University, Xuzhou 221116, China
Abstract
Black rot disease, caused by Ceratocystis fimbriata Ellis & Halsted, severely affects both plant growth and post-harvest storage of sweet potatoes. Invertase (INV) enzymes play essential roles in hydrolyzing sucrose into glucose and fructose and participate in the regulation of plant defense responses. However, little is known about the functions of INV in the growth and responses to black rot disease in sweet potato. In this study, we identified and characterized an INV-like gene, named IbINV, from sweet potato. IbINV contained a pectin methylesterase-conserved domain. IbINV transcripts were most abundant in the stem and were significantly induced in response to C. fimbriata, salicylic acid, and jasmonic acid treatments. Overexpressing IbINV in sweet potato (OEV plants) led to vigorous growth and high resistance to black rot disease, while the down-regulation of IbINV by RNA interference (RiV plants) resulted in reduced plant growth and high sensitivity to black rot disease. Furthermore, OEV plants contained a decreased sucrose content and increased hexoses content, which might be responsible for the increased INV activities; not surprisingly, RiV plants showed the opposite effects. Taken together, these results indicate that IbINV positively regulates plant growth and black rot disease resistance in sweet potato, mainly by modulating sugar metabolism.
Funder
National Natural Science Foundation of China China Agriculture Research System Jiangsu Government Scholarship for Oversea Studies Foundation of Xuzhou Academy of Agricultural Sciences Korea government
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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