Green Extraction and Preliminary Biological Activity of Hydrolyzed Collagen Peptides (HCPs) Obtained from Whole Undersized Unwanted Catches (Mugil cephalus L.)
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Published:2023-11-17
Issue:22
Volume:28
Page:7637
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ISSN:1420-3049
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Container-title:Molecules
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language:en
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Short-container-title:Molecules
Author:
Orlandi Valentina1, Dondero Lorenzo2, Turrini Federica13ORCID, De Negri Atanasio Giulia2, Grasso Federica1, Grasselli Elena234ORCID, Boggia Raffaella15ORCID
Affiliation:
1. Department of Pharmacy, University of Genova, Viale Cembrano 4, 16148 Genova, Italy 2. Department of Earth, Environmental and Life Sciences, University of Genova, Corso Europa, 26, 16132 Genova, Italy 3. National Center for the Development of New Technologies in Agriculture (Agritech), 80121 Napoli, Italy 4. Interuniversity Center for the Promotion of 3R Principles in Teaching and Research (Centro 3R), 56122 Pisa, Italy 5. National Biodiversity Future Center (NBFC), 90133 Palermo, Italy
Abstract
Considering the global increase in fish consumption, the growing side-streams coming from the fish supply chain (e.g., skin, fins, tail, heads…), also including undersized or “unwanted catches”, have been recently proposed as source of high-value bioactive compounds (e.g., peptides and fatty acids). In this case study, hydrolyzed collagen peptides (HCPs) were extracted from different parts of Mugil cephalus L. using environmentally friendly techniques such as ultrasounds and enzymatic treatments. Both a mixed biomass derived from the skin, fins, and tail, and a whole fish, were considered as starting biomass, simulating the unsorted processing side-streams and an undersized/unwanted catch, respectively. The extracted HCPs were purified in fractions (<3 KDa and >3 KDa) whose yields (about 5% and 0.04–0.3%, respectively) demonstrated the efficiency of the hydrolysis process. The extraction protocol proposed allowed us to also isolate the intermediate products, namely the lipids (about 8–10%) and the non-collagenous proteins (NCs, 16–23%), whose exploitation could be considered. Each sample was characterized using Sircol, UltraViolet-Spectra, and hydroxyproline assay, and the viability of their collagen fractions was tested on human endothelial cells. Significant effects were obtained at a fraction of <3 KDa, in particular at a concentration of 0.13 µg/mL. The T-scratch test was also performed, with positive results in all fractions tested.
Funder
Project National Center for the Development of New Technologies in Agriculture National Recovery and Resilience Plan Italian Ministry of University and Research European Union, NextGenerationEU Project National Biodiversity Future Center
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference59 articles.
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