Vasorelaxant Activity of Salvia hispanica L.: Involvement of the Nitric Oxide Pathway in Its Pharmacological Mechanism

Author:

Herbert-Doctor Luis A.1ORCID,Sánchez-Recillas Amanda1ORCID,Ortiz-Andrade Rolffy1ORCID,Hernández-Núñez Emanuel2ORCID,Araujo-León Jesús Alfredo3ORCID,Coral-Martínez Tania Isolina4ORCID,Cob-Calan Nubia Noemi5,Segura Campos Maira Rubi6ORCID,Estrada-Soto Samuel7ORCID

Affiliation:

1. Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán, Mérida 97069, Yucatan, Mexico

2. Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del IPN, Mérida 97310, Yucatán, Mexico

3. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Cinetífica de Yucatán, A.C., Mérida 97205, Yucatan, Mexico

4. Laboratorio de Cromatografía, Facultad de Química, Universidad Autónoma de Yucatán, Mérida 97069, Yucatan, Mexico

5. Instituto Tecnológico Superior de Calkiní en el Estado de Campeche, Calkiní 24900, Campeche, Mexico

6. Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Mérida 97203, Yucatán, Mexico

7. Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Morelos, Mexico

Abstract

Salvia hispanica L., commonly known as chía, and its seeds have been used since ancient times to prepare different beverages. Due to its nutritional content, it is considered a dietary ingredient and has been reported with many health benefits. Chia seed components are helpful in cardiovascular disease (CVD) by reducing blood pressure, platelet aggregation, cholesterol, and oxidation. Still, its vasodilator effects on the vascular system were not reported yet. The hexanic (HESh), dichloromethanic (DESh), and methanolic (MESh) extracts obtained from chía seeds were evaluated on an aortic ring ex-vivo experimental model. The vasorelaxant efficacy and mechanism of action were determined. Also, phytochemical data was obtained through 13C NMR-based dereplication. The MESh extract showed the highest efficacy (Emax = 87%), and its effect was partially endothelium-dependent. The mechanism of action was determined experimentally, and the vasorelaxant curves were modified in the presence of L-NAME, ODQ, and potassium channel blockers. MESh caused a relaxing effect on KCl 80 mM-induced contraction and was less potent than nifedipine. The CaCl2-induced contraction was significantly decreased compared with the control curve. Phytochemical analysis of MESh suggests the presence of mannitol, previously reported as a vasodilator on aortic rings. Our findings suggest NO-cGMP pathway participation as a vasodilator mechanism of action of S. hispanica seeds; this effect can be attributed, in part, to the mannitol presence. S. hispanica could be used in future research focused on antihypertensive therapies.

Funder

CYTED-Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo

Publisher

MDPI AG

Subject

Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science

Reference45 articles.

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