Effects of Different pH Levels on the Structural and Functional Properties of Proteins of Phaeodactylum tricornutum

Author:

Wang Yanli1ORCID,Zhu Laijing1ORCID,Zhu Zhunyao1,Liu Meng1,Zhao Xiangzhong1ORCID

Affiliation:

1. School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China

Abstract

Phaeodactylum tricornutum is identified by its capacity for rapid growth, reproduction, and in vitro cultivation, as well as the presence of a range of high-value active compounds, including proteins, with potential food applications. The objective of this study was to investigate the effects of pH shift treatments (pH of 3, 5, 7, 9, and 11) on the structural and functional properties of the Phaeodactylum tricornutum protein (PTP). The molecular weight of the PTP was predominantly distributed within the following ranges: below 5 kDa, 5–100 kDa, and above 100 kDa. Compared to the acidic environment, the PTP demonstrated higher solubility and greater free sulfhydryl group content in the alkaline environment. Additionally, PTP had a smaller particle size and higher thermal stability in alkaline environments. The PTP exhibited superior foaming ability (135%), emulsification activity index (3.72 m2/g), and emulsion stability index (137.71 min) in alkaline environments. The results of this investigation provide a foundation for the future development and application of the PTP in the food industry.

Funder

“reveal the list system” science and technology project tasks, Rongcheng, China

Key R&D Program of Shandong Province, China

Publisher

MDPI AG

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