Beneficial Effects of Dietary Fiber in Young Barley Leaf on Gut Microbiota and Immunity in Mice

Author:

Chudan Seita1,Kurakawa Takuto1,Nishikawa Miyu1,Nagai Yoshinori2ORCID,Tabuchi Yoshiaki3ORCID,Ikushiro Shinichi1ORCID,Furusawa Yukihiro23ORCID

Affiliation:

1. Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Toyama 939-0398, Japan

2. Department of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, Toyama 939-0398, Japan

3. Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Toyama 930-0194, Japan

Abstract

The health benefits of young barley leaves, rich in dietary fiber, have been studied for several decades; however, their beneficial effects on the intestinal microenvironment remain to be elucidated. To investigate the effects of young barley leaf-derived dietary fiber (YB) on the gut microbiota and immunity, mice were fed an AIN-93G diet containing cellulose or YB and subjected to subsequent analysis. The population of MHC-II-positive conventional dendritic cells (cDCs) and CD86 expression in the cDCs of Peyer’s patches were elevated in the YB-fed mice. MHC-II and CD86 expression was also elevated in the bone marrow-derived DCs treated with YB. 16S-based metagenomic analysis revealed that the gut microbiota composition was markedly altered by YB feeding. Among the gut microbiota, Lachnospiraceae, mainly comprising butyrate-producing NK4A136 spp., were overrepresented in the YB-fed mice. In fact, fecal butyrate concentration was also augmented in the YB-fed mice, which coincided with increased retinaldehyde dehydrogenase (RALDH) activity in the CD103+ cDCs of the mesenteric lymph nodes. Consistent with elevated RALDH activity, the population of colonic IgA+ plasma cells was higher in the YB-fed mice than in the parental control mice. In conclusion, YB has beneficial effects on the gut microbiota and intestinal immune system.

Funder

JSPS KAKENHI

Takeda Science Foundation

Iijima Tojuro Foundation

Publisher

MDPI AG

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