Distribution, Typical Structure and Self-Assembly Properties of Collagen from Fish Skin and Bone
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Published:2023-09-08
Issue:18
Volume:28
Page:6529
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ISSN:1420-3049
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Container-title:Molecules
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language:en
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Short-container-title:Molecules
Author:
Zhang Xuening1, Wang Jie1, Zhang Qian1, Fan Yan2, Zhang Hongwei3, Ahmad Khurshid1ORCID, Hou Hu1456
Affiliation:
1. College of Food Science and Engineering, Ocean University of China, Sansha Road, Qingdao 266404, China 2. College of Marine Life Sciences, Ocean University of China, Yushan Road, Qingdao 266003, China 3. Technology Center of Qingdao Customs, Xinyue Road, Qingdao 266109, China 4. Laboratory for Marine Drugs and Bioproducts, Laoshan Laboratory, Qingdao 266237, China 5. Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, China 6. Qingdao Institute of Marine Bioresources for Nutrition and Health Innovation, Qingdao 266000, China
Abstract
The source and type of collagen are crucial to its application, and both play a decisive role. Collagen was prepared from both tilapia skin and bone and skate skin and cartilage, named as CI-TI-s, CI-TI-b, CI-SK-s, and CII-SK-c, respectively. Types, distributions, structures, and self-assembly of collagen were studied. It showed that yellow collagen fibers from skin arranged longitudinally, while collagen fibers from skate cartilages displayed varying colors. CI-TI-s, CI-TI-b, CI-SK-s, and CII-SK-c showed the typical amide A (3316–3336 cm−1) and amide B (2929–2948 cm−1) in FTIR spectra. CI-TI-b and CII-SK-c showed 218–229 nm of UV absorption, 11.56–12.20 Å of d values in XRD, and 0.12–0.14 of Rpn values in CD. The thermal denaturation temperatures of CI-TI-s and CI-SK-s were 30.7 and 20.6 °C, respectively. The self-assembly of CI-TI-s and CII-SK-c were maximum at pH 7.2 and 7.4–7.6, respectively. The unique collagen peptides of tilapia and skate were GPSGPQGAVGATGPK, PAMPVPGPMGPMGPR, SPAMPVPGPMGPMGPR, GESGPSGPAGPAGPAGVR, SSGPPVPGPIGPMGPR, GLTGPIGVPGPPGAQGEK, GLAGPQGPR, and GLSGDPGVQGIK, respectively. The unique peptides of type I and type II collagen were GPTGEIGATGLAGAR, GVLGLTGMR, LGLTGMR, GEPGAAGPAGPSGPMGPR, SSGPPVPGPIGPMGPR, and GLSGDPGVQGIK, respectively.
Funder
Major Scientific and Technological Innovation Project in Shandong Province Major Scientific and Technological Innovation Project of Qingdao West Coast New Area Taishan Scholar Foundation of Shandong Province Marine S&T Fund of Shandong Province for Qingdao Marine Science and Technology Center
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
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