Nature-Inspired Biomolecular Corona Based on Poly(caffeic acid) as a Low Potential and Time-Stable Glucose Biosensor
-
Published:2023-10-26
Issue:21
Volume:28
Page:7281
-
ISSN:1420-3049
-
Container-title:Molecules
-
language:en
-
Short-container-title:Molecules
Author:
Kuznowicz Maria1, Jędrzak Artur1, Jesionowski Teofil1ORCID
Affiliation:
1. Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland
Abstract
Herein, we present a novel biosensor based on nature-inspired poly(caffeic acid) (PCA) grafted to magnetite (Fe3O4) nanoparticles with glucose oxidase (GOx) from Aspergillus niger via adsorption technique. The biomolecular corona was applied to the fabrication of a biosensor system with a screen-printed electrode (SPE). The obtained results indicated the operation of the system at a low potential (0.1 V). Then, amperometric measurements were performed to optimize conditions like various pH and temperatures. The SPE/Fe3O4@PCA-GOx biosensor presented a linear range from 0.05 mM to 25.0 mM, with a sensitivity of 1198.0 μA mM−1 cm−2 and a limit of detection of 5.23 μM, which was compared to other biosensors presented in the literature. The proposed system was selective towards various interferents (maltose, saccharose, fructose, L-cysteine, uric acid, dopamine and ascorbic acid) and shows high recovery in relation to tests on real samples, up to 10 months of work stability. Moreover, the Fe3O4@PCA-GOx biomolecular corona has been characterized using various techniques such as Fourier transform infrared spectroscopy (FTIR), high-resolution transmission electron microscopy (HRTEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and Bradford assay.
Funder
National Science Centre Poland Foundation for Polish Science
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference45 articles.
1. Cheng, Y., Gong, X., Yang, J., Zheng, G., Zheng, Y., Li, Y., Xu, Y., Nie, G., Xie, X., and Chen, M. (2022). A Touch-Actuated Glucose Sensor Fully Integrated with Microneedle Array and Reverse Iontophoresis for Diabetes Monitoring. Biosens. Bioelectron., 203. 2. Diabetes Mellitus: The Epidemic of the Century;Kharroubi;World J. Diabetes,2015 3. Glucose Biosensors for Clinical and Personal Use;Karyakin;Electrochem. Commun.,2021 4. Jędrzak, A., Kuznowicz, M., Rębiś, T., and Jesionowski, T. (2022). Portable Glucose Biosensor Based on Polynorepinephrine@magnetite Nanomaterial Integrated with a Smartphone Analyzer for Point-of-Care Application. Bioelectrochemistry, 145. 5. Mobile-assisted diagnostic biosensor for point-of-care glucose detection in real human samples with rapid response and long-live stability;Kuznowicz;J. Appl. Electrochem.,2023
|
|