Effect of the Harvest Season of Anthyllis henoniana Stems on Antioxidant and Antimicrobial Activities: Phytochemical Profiling of Their Ethyl Acetate Extracts
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Published:2023-05-07
Issue:9
Volume:28
Page:3947
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ISSN:1420-3049
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Container-title:Molecules
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language:en
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Short-container-title:Molecules
Author:
Ayachi Amani1, Ben Younes Amer1, Ben Ammar Ameni2, Bouzayani Bouthaina1, Samet Sonda1, Siala Mariam2, Trigui Mohamed2, Treilhou Michel3, Téné Nathan3ORCID, Mezghani-Jarraya Raoudha1
Affiliation:
1. Laboratory of Organic Chemistry LR17ES08, Natural Substances Team, Faculty of Sciences of Sfax, University of Sfax, P.B 1171, Sfax 3000, Tunisia 2. Laboratory of Environmental Sciences and Sustainable Development “LASED”, Sfax Preparatory Institute for Engineering Studies, University of Sfax, P.B 1171, Sfax 3000,Tunisia 3. Equipe BTSB-EA 7417, Institut National Universitaire Jean-François Champollion, Université de Toulouse, Place de Verdun, 81012 Albi, France
Abstract
Anthyllis henoniana stems were harvested in two seasons: winter and spring (February and May 2021). In this study, we investigated the antioxidant (DPPH, ABTS, FRAP and TAC) and antimicrobial activities, total phenolic contents and total flavonoid contents of the obtained extracts (hexane, ethyl acetate and methanol). The results showed that ethyl acetate extract from stems harvested in winter exhibited the highest antioxidant activity, while ethyl acetate extract from the stems harvested in spring showed the most potent antibacterial and antifungal activities. To explain these differences, we investigated the phytochemical composition of these two extracts using liquid chromatography coupled with mass spectrometry. Therefore, 45 compounds were detected, from which we identified 20 compounds (flavonoids, triterpenoids, chalcones and phenolic acids); some were specific to the harvest month while others were common for both periods. Some of the major compounds detected in ethyl acetate (spring) were dihydrochalcone (Kanzonol Y, 8.2%) and flavanone (sophoraflavanone G, 5.9%), previously recognized for their antimicrobial effects. We therefore concluded that the difference in activities observed for the two harvest periods depends on the chemical composition of the extracts and the relative abundance of each compound.
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
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