In Vitro Neurotrophic Properties and Structural Characterization of a New Polysaccharide LTC-1 from Pyrola corbieri Levl (Luticao)
-
Published:2023-02-06
Issue:4
Volume:28
Page:1544
-
ISSN:1420-3049
-
Container-title:Molecules
-
language:en
-
Short-container-title:Molecules
Author:
Li Liangqun12, Yu Kangkang3, Mo Zhengchang4, Yang Keling12, Chen Fuxue3, Yang Juan12ORCID
Affiliation:
1. State Key Laboratory for Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China 2. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China 3. School of Life Science, Shanghai University, Shanghai 200444, China 4. Tongren Polytechnic College, Tongren 554300, China
Abstract
Pyrola corbieri Levl has been used to strengthen bones and nourish the kidney (the kidney governs the bone and is beneficial to the brain) by the local Miao people in China. However, the functional components and neurotrophic activity have not been reported. A new acidic homogeneous heteropolysaccharide named LTC-1 was obtained and characterized by periodate oxidation, Smith degradation, partial acid hydrolysis, GC–MS spectrometry, methylation analysis, and Fourier transform infrared spectroscopy, and its molecular weight was 3239 Da. The content of mannuronic acid (Man A) in LTC-1 was 46%, and the neutral sugar was composed of L-rhamnose (L-Rha), L-arabinose (L-Ara), D-xylose (D-Xyl), D-mannose (D-Man), D-glucose (D-Glc) and D-galactose (D-Gal) with a molar ratio of 1.00:3.63:0.86:1.30:6.97:1.30. The main chain of LTC-1 was composed of Glc, Gal, Man, Man A and the branched chain Ara, Glc, Gal. The terminal residues were composed of Glc and Gal. The main chain and branched chains were linked by (1→5)-linked-Ara, (1→3)-linked-Glc, (1→4)-linked-Glc, (1→6)-linked-Glc, (1→3)-linked-Gal, (1→6)-linked-Gal, (1→3, 6)-linked-Man and ManA. Meanwhile, neurotrophic activity was evaluated through PC12 and primary hippocampal neuronal cell models. LTC-1 exhibited neurotrophic activity in a concentration-dependent manner, which significantly induced the differentiation of PC12 cells, promoted the neurite outgrowth of PC12 cells, enhanced the formation of the web architecture of dendrites, and increased the density of dendritic spines in hippocampal neurons and the expression of PSD-95. These results displayed significant neurotrophic factor-like activity of LTC-1, which suggests that LTC-1 is a potential treatment option for neurodegenerative diseases.
Funder
National Natural Science Foundation of China Guizhou Provincial Science and Technology Foundation Guizhou Province Innovation Capability Improvement Platform Guizhou Province Engineering Research Center for Efficient Utilization of Natural Products
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference44 articles.
1. Punicalagin attenuates LPS-induced inflammation and ROS production in microglia by inhibiting the MAPK/NF-κB signaling pathway and NLRP3 inflammasome activation;Lo;J. Inflamm. Res.,2022 2. Ismail, M., Alsalahi, A., Imam, M.U., Ooi, D.J.H., Khaza’ai, M.A., Aljaberi, M.N., and Shamsudin, Z. (2020). IdrusSafety and neuroprotective efficacy of palm oil and tocotrienol-rich fraction from palm oil: A systematic review. Nutrients, 12. 3. Association between BDNF gene variant Rs6265 and the severity of depression in antidepressant treatment-free depressed patients;Losenkov;Front. Psychiatry,2020 4. Andrea, M., Margrate, A., Marinella, C., Giulia, A., Sara, A.B., Gregorio, P., Emanuela, T., Mariachiara, P., Giovanni, R., and Erika, O. (2023). The bright side of psychedelics: Latest advances and challenges in neuropharmacology. Int. J. Mol. Sci., 24. 5. Kim, A., Jung, H.G., Kim, Y.E., Kim, S.C., Park, J.Y., Lee, S.G., and Hwang, E.M. (2019). The knockdown of TREK-1 in hippocampal neurons attenuate lipopolysaccharide-induced depressive-like behavior in mice. Int. J. Mol. Sci., 20.
|
|