Discrimination of Radix Astragali from Different Growth Patterns, Origins, Species, and Growth Years by an H1-NMR Spectrogram of Polysaccharide Analysis Combined with Chemical Pattern Recognition and Determination of Its Polysaccharide Content and Immunological Activity

Author:

Guo Yali1,Wang Bing2,Gu Lifei2,Yin Guo2,Wang Shuhong2,Li Meifang2,Wang Lijun2,Yu Xie-An2ORCID,Wang Tiejie12

Affiliation:

1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China

2. NMPA Key Laboratory for Quality Research and Evaluation of Traditional Chinese Medicine, Shenzhen Institute for Drug Control, Shenzhen 518057, China

Abstract

The fraud phenomenon is currently widespread in the traditional Chinese medicine Radix Astragali (RA) market, especially where high-quality RA is substituted with low-quality RA. In this case, focused on polysaccharides from RA, the classification models were established for discrimination of RA from different growth patterns, origins, species, and growth years. 1H Nuclear Magnetic Resonance (H1-NMR) was used to establish the spectroscopy of polysaccharides from RA, which were used to distinguish RA via chemical pattern recognition methods. Specifically, orthogonal partial least squares discriminant analysis (OPLS-DA) and linear discriminant analysis (LDA) were used to successfully establish the classification models for RA from different growth patterns, origins, species, and growth years. The satisfactory parameters and high accuracy of internal and external verification of each model exhibited the reliable and good prediction ability of the developed models. In addition, the polysaccharide content and immunological activity were also tested, which was evaluated by the phagocytic activity of RAW 264.7. And the result showed that growth patterns and origins significantly affected the quality of RA. However, there was no significant difference in the aspects of origins and growth years. Accordingly, the developed strategy combined with chemical information, biological activity, and multivariate statistical method can provide new insight for the quality evaluation of traditional Chinese medicine.

Funder

Special Project for Sustainable Development of Shenzhen Science and Technology Innovation Committee

Publisher

MDPI AG

Subject

Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science

Reference37 articles.

1. Quality control of Radix astragali (the root of Astragalus membranaceus var. mongholicus) along its value chains;Bi;Front. Pharmacol.,2020

2. Application of Invigorating Qi and Moistening Intestines Method in Qi Deficiency Constipation;Li;Int. J. Clin. Exp. Med. Res.,2022

3. Chinese Pharmacopoeia Commission (2020). Pharmacopoeia of the People’s Republic of China, China Medicine Science and Technology Press.

4. Effect of Shenqi Fuzheng Injection on leukopenia and T-cell subsets in patients with non-small cell lung cancer undergoing radiotherapy;Wu;Evid. Based Complement. Altern. Med.,2022

5. Anti-tumor activity of the fermentation broth of Cordyceps militaris cultured in the medium of Radix astragali;Lin;Process Biochem.,2008

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3