In Vitro Cytotoxic Effects and Mechanisms of Action of Eleutherine Isolated from Eleutherine plicata Bulb in Rat Glioma C6 Cells
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Published:2022-12-13
Issue:24
Volume:27
Page:8850
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ISSN:1420-3049
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Container-title:Molecules
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language:en
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Short-container-title:Molecules
Author:
Shinkai Victoria Mae Tsuruzaki, Sampaio Izana Marize Oliveira, dos Santos Eline Gomes, Galué-Parra Adan JesúsORCID, Ferreira Dionisia Pelaes, Baliza Drielly Dayanne Monteiro SantosORCID, Ramos Neidiane Farias, Pimenta Raphael SanzioORCID, Burbano Rommel Mario RodriguezORCID, Sena Chubert Bernardo CastroORCID, Macchi Barbarella MatosORCID, Ferreira Irlon MacielORCID, Silva Edilene OliveiraORCID, do Nascimento José Luiz Martins
Abstract
Gliomas are the most common primary malignant brain tumors in adults, and have a poor prognosis, despite the different types of treatment available. There is growing demand for new therapies to treat this life-threatening tumor. Quinone derivatives from plants have received increased interest as potential anti-glioma drugs, due to their diverse pharmacologic activities, such as inhibiting cell growth, inflammation, tumor invasion, and promoting tumor regression. Previous studies have demonstrated the anti-glioma activity of Eleutherine plicata, which is related to three main naphthoquinone compounds—eleutherine, isoeleutherine, and eleutherol—but their mechanism of action remains elusive. Thus, the aim of this study was to investigate the mechanism of action of eleutherine on rat C6 glioma. In vitro cytotoxicity was evaluated by MTT assay; morphological changes were evaluated by phase-contrast microscopy. Apoptosis was determined by annexin V–FITC–propidium iodide staining, and antiproliferative effects were assessed by wound migration and colony formation assays. Protein kinase B (AKT/pAKT) expression was measured by western blot, and telomerase reverse transcriptase mRNA was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Eleutherine reduced C6 cell proliferation in a dose-dependent manner, suppressed migration and invasion, induced apoptosis, and reduced AKT phosphorylation and telomerase expression. In summary, our results suggest that eleutherine has potential clinical use in treating glioma.
Funder
Conselho Nacional de Desenvolvimento Científico e Tecnológico Fundação de Amparo à Pesquisa do Estado do Pará Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Pró-Reitoria de Pesquisa e Pós-graduação
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference50 articles.
1. Epidemiology and molecular pathology of glioma;Schwartzbaum;Nat. Clin. Pract. Neurol.,2006 2. Malignant gliomas in adults;Wen;N. Engl. J. Med.,2008 3. Glioblastoma: From Molecular Pathology to Targeted Treatment;Cloughesy;Annu. Rev. Pathol.,2014 4. Chen, D.L., Hu, M.G., liu, Y.Y., Li, R.T., Yu, M., Xu, X.D., and Ma, G.X. (2018). New Naphthalene Derivatives from the Bulbs of Eleutherine americana with Their Protective Effect on the Injury of HUVECs. Molecules, 23. 5. Chokshi, C.R., Brakel, B.A., Tatari, N., Savage, N., Salim, S.K., Venugopal, C., and Singh, S.K. (2021). Advances in Immunotherapy for Adult Glioblastoma. Cancers, 13.
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