Phytochemical Profiles and Antimicrobial Activity of Selected Populus spp. Bud Extracts
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Published:2024-01-16
Issue:2
Volume:29
Page:437
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ISSN:1420-3049
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Container-title:Molecules
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language:en
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Short-container-title:Molecules
Author:
Okińczyc Piotr1ORCID, Widelski Jarosław2ORCID, Nowak Kinga3ORCID, Radwan Sylwia4, Włodarczyk Maciej1ORCID, Kuś Piotr Marek1ORCID, Susniak Katarzyna5ORCID, Korona-Głowniak Izabela5ORCID
Affiliation:
1. Department of Pharmacognosy and Herbal Medicines, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211a, PL-50-556 Wrocław, Poland 2. Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Lublin, Chodźki 1, PL-20-093 Lublin, Poland 3. Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, PL-62-035 Kórnik, Poland 4. Laboratory of Elemental Analysis and Structural Research, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211a, PL-50-556 Wrocław, Poland 5. Department of Pharmaceutical Microbiology, Medical University of Lublin, Chodźki 1, PL-20-093 Lublin, Poland
Abstract
Buds of poplar trees (Populus species) are often covered with sticky, usually polyphenol-rich, exudates. Moreover, accessible data showed that some Populus bud extracts may be excellent antibacterial agents, especially against Gram-positive bacteria. Due to the fragmentary nature of the data found, we conducted a systematic screening study. The antimicrobial activity of two extract types (semi-polar—ethanolic and polar—ethanolic-water (50/50; V/V)) from 27 bud samples of different poplar taxons were compared. Antimicrobial assays were performed against Gram-positive (five strains) and Gram-negative (six strains) bacteria as well as fungi (three strains) and covered the determination of minimal inhibitory, bactericidal, and fungicidal concentrations. The composition of extracts was later investigated by ultra-high-performance liquid chromatography coupled with ultraviolet detection (UHPLC-DAD) and with electrospray-quadrupole-time-of-flight tandem mass spectrometry (UHPLC-ESI-qTOF-MS). As a result, most of the extracts exhibited good (MIC ≤ 62.5 µg/mL) or moderate (62.5 < MIC ≤ 500 µg/mL) activity against Gram-positives and Helicobacter pylori, as well as fungi. The most active were ethanolic extracts from P. trichocarpa, P. trichocarpa clone ‘Robusta’, and P. tacamahaca × P. trichocarpa. The strongest activity was observed for P. tacamahaca × P. trichocarpa. Antibacterial activity was supposedly connected with the abundant presence of flavonoids (pinobanksin, pinobanksin 3-acetate, chrysin, pinocembrin, galangin, isosakuranetin dihydrochalcone, pinocembrin dihydrochalcone, and 2′,6′-dihydroxy-4′-methoxydihydrochalcone), hydroxycinnamic acids monoesters (p-methoxycinnamic acid cinnamyl ester, caffeic acid phenethylate and different isomers of prenyl esters), and some minor components (balsacones).
Funder
Wroclaw Medical University Medical University of Lublin Institute of Dendrology
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
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