Biomolecular Screening of Pimpinella anisum L. for Antioxidant and Anticholinesterase Activity in Mice Brain

Author:

Mushtaq Aamir1ORCID,Habib Fatima2,Manea Rosana3ORCID,Anwar Rukhsana4,Gohar Umar Farooq5ORCID,Zia-Ul-Haq Muhammad6,Ahmad Mobasher2,Gavris Claudia Mihaela3ORCID,Chicea Liana7

Affiliation:

1. Department of Pharmaceutical Sciences, Government College University, Lahore 54000, Pakistan

2. Gulab Devi Institute of Pharmacy, Gulab Devi Educational Complex, Lahore 54000, Pakistan

3. Faculty of Medicine, Transilvania University of Brasov, 500036 Brasov, Romania

4. Department of Pharmacology, Punjab University College of Pharmacy, University of the Punjab, Lahore 54000, Pakistan

5. Institute of Industrial Biotechnology, Government College University, Lahore 54000, Pakistan

6. Office of Research, Innovation and Commercialization, Lahore College for Women University, Lahore 54000, Pakistan

7. Faculty of Medicine, University “Lucian Blaga” Sibiu, 550169 Sibiu, Romania

Abstract

Hundreds of the plants have been explored and evaluated for antioxidant and anti-amnesic activities, so far. This study was designed to report the biomolecules of Pimpinella anisum L. for the said activities. The aqueous extract of dried P. anisum seeds was fractionated via column chromatography and the fractions so obtained were assessed for the inhibition of acetylcholinesterase (AChE) via in vitro analysis. The fraction which best inhibited AChE was so named as the P. anisum active fraction (P.aAF). The P.aAF was then chemically analyzed via GCMS, which indicated that oxadiazole compounds were present in it. The P.aAF was then administered to albino mice to conduct the in vivo (behavioral and biochemical) studies. The results of the behavioral studies indicated the significant (p < 0.001) increase in inflexion ratio, by the number of hole-pokings through holes and time spent in a dark area by P.aAF treated mice. Biochemical studies demonstrated that the oxadiazole present in P.aAF on one hand presented a noteworthy reduction in MDA and the AChE level and on the other hand promoted the levels of CAT, SOD and GSH in mice brain. The LD50 for P.aAF was calculated as 95 mg/Kg/p.o. The findings thus supported that the antioxidant and anticholinesterase activities of P. anisum are due to its oxadiazole compounds.

Publisher

MDPI AG

Subject

Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science

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