Targeted HPTLC Profile, Quantification of Flavonoids and Phenolic Acids, and Antimicrobial Activity of Dodonaea angustifolia (L.f.) Leaves and Flowers

Author:

Tessema Fekade Beshah123ORCID,Gonfa Yilma Hunde124ORCID,Asfaw Tilahun Belayneh15ORCID,Tadesse Mesfin Getachew12ORCID,Tadesse Tigist Getachew6,Bachheti Archana7,Alshaharni Mohammed O.8,Kumar Pankaj9ORCID,Kumar Vinod9ORCID,Širić Ivan10ORCID,Abou Fayssal Sami1112ORCID,Chaubey Kundan Kumar13ORCID,Bachheti Rakesh Kumar12ORCID

Affiliation:

1. Department of Industrial Chemistry, Addis Ababa Science and Technology University, Addis Ababa P.O. Box 16417, Ethiopia

2. Centre of Excellence in Biotechnology and Bioprocess, Addis Ababa Science and Technology University, Addis Ababa P.O. Box 16417, Ethiopia

3. Department of Chemistry, Faculty of Natural and Computational Science, Woldia University, Woldia P.O. Box. 400, Ethiopia

4. Department of Chemistry, Faculty of Natural and Computational Science, Ambo University, Ambo P.O. Box 19, Ethiopia

5. Department of Chemistry, College of Natural and Computational Science, Gondar University, Gondar P.O. Box 196, Ethiopia

6. Bio and Emerging Technology Institute, Health Biotechnology Directorate, Addis Ababa P.O. Box 5954, Ethiopia

7. Department of Environment Science, Graphic Era (Deemed to be University), Dehradun 248002, India

8. Department of Biology, College of Science, King Khalid University, Abha 61321, Saudi Arabia

9. Agro-Ecology and Pollution Research Laboratory, Department of Zoology and Environmental Science, Gurukula Kangri (Deemed to Be University), Haridwar 249404, India

10. University of Zagreb, Faculty of Agriculture, Svetosimunska 25, 10000 Zagreb, Croatia

11. Department of Agronomy, Faculty of Agronomy, University of Forestry, 10 Kliment Ohridski Blvd, 1797 Sofia, Bulgaria

12. Department of Plant Production, Faculty of Agriculture, Lebanese University, Beirut 1302, Lebanon

13. Division of Research and Innovation, School of Applied and Life Sciences, Uttaranchal University, Arcadia Grant, P.O. Chandanwari, Prem Nagar, Dehradun 248007, India

Abstract

In East Africa, Dodonaea angustifolia (L.f.) is a well-known medicinal herb. Its leaf is primarily studied in light of its ethnobotanical use. In terms of phytochemistry and biological activity, its flower is not studied. In a prior study, our team looked into phytochemical screening, antioxidant activity, and total phenolic levels. This study aims to compare the profiles and biological activities of the leaf and flower samples of D. angustifolia and to present therapeutic alternatives. The leaf and flower sample powders were extracted with methanol using ultrasound-assisted extraction (UAE). HPTLC profile was obtained using CAMAG—HPTLC equipped with VisionCATS software. Antimicrobial agar well diffusion assay and minimum inhibition concentration (MIC) were determined. The leaf and flower extracts of D. angustifolia showed antibacterial activity with a MIC value of 20 µg/mL against Enterococcus faecalis and Listeria monocytogenes. Similarly, 40 µg/mL was found to be effective against Aspergillus flavus. D. angustifolia flower is a rich source of flavonoids and phenolic acids. Because of its antibacterial properties and profile, which are almost the same, the flower is emerging as a viable option for medicinal alternatives.

Funder

Deanship of Scientific Research at King Khalid University

Ph.D. dissertation grant from Addis Ababa Science and Technology University

Publisher

MDPI AG

Subject

Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science

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