Ecotoxicity of Diazinon and Atrazine Mixtures after Ozonation Catalyzed by Na+ and Fe2+ Exchanged Montmorillonites on Lemna minor

Abstract

The toxicity of two pesticides, diazinon (DAZ) and atrazine (ATR), before and after montmorillonite-catalyzed ozonation was comparatively investigated on the duckweed Lemna minor. The results allowed demonstrating the role of clay-containing media in the evolution in time of pesticide negative impact on L. minor plants. Pesticides conversion exceeded 94% after 30 min of ozonation in the presence of both Na+ and Fe2+ exchanged montmorillonites. Toxicity testing using L. minor permitted us to evaluate the change in pesticide ecotoxicity. The plant growth inhibition involved excessive oxidative stress depending on the pesticide concentration, molecular structure, and degradation degree. Pesticide adsorption and/or conversion by ozonation on clay surfaces significantly reduced the toxicity towards L. minor plants, more particularly in the presence of Fe(II)-exchanged montmorillonite. The results showed a strong correlation between the pesticide toxicity towards L. minor and the level of reactive oxygen species, which was found to depend on the catalytic activity of the clay minerals, pesticide exposure time to ozone, and formation of harmful derivatives. These findings open promising prospects for developing a method to monitor pesticide ecotoxicity according to clay-containing host-media and exposure time to ambient factors.