Affiliation:
1. Department of Industrial Chemistry “Toso Montanari”, Alma Mater Studiorum-University of Bologna, Viale del Risorgimento 4, 40136 Bologna, Italy
Abstract
Due to the growing presence of pesticides in the environment and in food, the concern of their impact on human health is increasing. Therefore, the development of fast and reliable detection methods is needed. Enzymatic inhibition-based biosensors represent a good alternative for replacing the more complicated and time-consuming traditional methods (chromatography, spectrophotometry, etc.). This paper describes the development of an electrochemical biosensor exploiting alkaline phosphatase as the biological recognition element and a chemically modified glassy carbon electrode as the transducer. The biosensor was prepared modifying the GCE surface by a mixture of Multi-Walled-Carbon-Nanotubes (MWCNTs) and Electrochemically-Reduced-Graphene-Oxide (ERGO) followed by the immobilization of the enzyme by cross-linking with bovine serum albumin and glutaraldehyde. The inhibition of the biosensor response caused by pesticides was established using 2-phospho-L-ascorbic acid as the enzymatic substrate, whose dephosphorylation reaction produces ascorbic acid (AA). The MWCNTs/ERGO mixture shows a synergic effect in terms of increased sensitivity and decreased overpotential for AA oxidation. The response of the biosensor to the herbicide 2,4-dichloro-phenoxy-acetic-acid was evaluated and resulted in the concentration range 0.04–24 nM, with a limit of the detection of 16 pM. The determination of other pesticides was also achieved. The re-usability of the electrode was demonstrated by performing a washing procedure.
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference38 articles.
1. Natural vs synthetic auxin: Studies on the interactions between plant hormones and biological membrane lipids;Environ. Res.,2014
2. (2023, January 05). 2,4 D. Available online: https://www.invasive.org/gist/products/handbook/10.24-D.pdf.
3. Environmental Protection Agency (EPA) (2007). Reregistration Eligibility Decision for Aliphatic Alcohols Reregistration Eligibility Decision for Aliphatic Alcohols, Environmental Protection Agency (EPA).
4. Auxin-type herbicide drift: Effects on grapevine leaf functioning and reproductive performance;Rossouw;IVES Tech. Rev. Vine Wine,2021
5. Peterson, M.A., Mcmaster, S.A., Riechers, D.E., Skelton, J., and Stahlman, P.W. (2016). 2,4-D Past, Present, and Future: A Review, Cambridge University Press.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献