Affiliation:
1. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China
2. Key Laboratory of Corn Deep Processing Theory and Technology of Heilongjiang Province, College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, China
Abstract
Alcohol dehydrogenase (ADH) plays a pivotal role in constraining alcohol metabolism. Assessing the ADH-activating activity in vitro can provide insight into the capacity to accelerate ethanol metabolism in vivo. In this study, ADH-activating peptides were prepared from corn protein meal (CGM) using enzymatic hydrolysis, and these peptides were subsequently identified following simulated gastrointestinal digestion and their absorption through the Caco-2 cell monolayer membrane. The current investigation revealed that corn protein hydrolysate hydrolyzed using alcalase exhibited the highest ADH activation capability, maintaining an ADH activation rate of 52.93 ± 2.07% following simulated gastrointestinal digestion in vitro. After absorption through the Caco-2 cell monolayer membrane, ADH-activating peptides were identified. Among them, SSNCQPF, TGCPVLQ, and QPQQPW were validated to possess strong ADH activation activity, with EC50 values of 1.35 ± 0.22 mM, 2.26 ± 0.16 mM, and 2.73 ± 0.13 mM, respectively. Molecular Docking revealed that the activation of ADH occurred via the formation of a stable complex between the peptide and the active center of ADH by hydrogen bonds and hydrophobic interactions. The results of this study also suggest that corn protein hydrolysate could be a novel functional dietary element that helps protects the liver from damage caused by alcohol and aids in alcohol metabolism.
Funder
National Key Research and Development Project of China
China Central Government Guided Heilongjiang Provincial Local Science and Technology Development Program
Innovation Scientific Research Fund for Graduate Students Harbin University of Commerce
Fundamental Research Funds of Department of Education of Heilongjiang Province
Heilongjiang Key Laboratory of Corn Deep Processing Theory and Technology
Reference40 articles.
1. National Bureau of Statistics of China (2023, December 25). Grain Yield Statistics Reports for the China. 2023 [EB/OL], Available online: https://www.stats.gov.cn/sj/zxfb/202312/t20231211_1945417.html.
2. Global maize production, utilization, and consumption;Ranum;Ann. N. Y. Acad. Sci.,2014
3. Mapping cropland suitability in China using optimized MaxEnt model;Li;Field Crop. Res.,2023
4. Fibrous coproducts of corn and citrus as forage and concentrate sources for dairy cows;Silva;J. Dairy Sci.,2022
5. Effect of rumen-protected lysine supplementation of diets based on corn protein fed to lactating dairy cows;Lobos;J. Dairy Sci.,2021