Affiliation:
1. Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs, Haikou Key Laboratory of Li Nationality Medicine, School of Pharmacy, Hainan Medical University, Haikou 571199, China
2. Hepatobiliary and Liver Transplantation Department of Hainan Digestive Disease Center, The Second Affiliated Hospital of Hainan Medical University, Haikou 570311, China
Abstract
AHP-3a, a triple-helix acidic polysaccharide isolated from Alpinia officinarum Hance, was evaluated for its anticancer and antioxidant activities. The physicochemical properties and structure of AHP-3a were investigated through gel permeation chromatography, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. The weight-average molecular weight of AHP-3a was 484 kDa, with the molar percentages of GalA, Gal, Ara, Xyl, Rha, Glc, GlcA, and Fuc being 35.4%, 21.4%, 16.9%, 11.8%, 8.9%, 3.1%, 2.0%, and 0.5%, respectively. Based on the results of the monosaccharide composition analysis, methylation analysis, and NMR spectroscopy, the main chain of AHP-3a was presumed to consist of (1→4)-α-D-GalpA and (1→2)-α-L-Rhap residues, which is a pectic polysaccharide with homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) structural domains containing side chains. In addition, the results of the antioxidant activity assay revealed that the ability of AHP-3a to scavenge DPPH, ABTS, and OH free radicals increased with an increase in its concentration. Moreover, according to the results from the EdU, wound healing, and Transwell assays, AHP-3a can control the proliferation, migration, and invasion of HepG2 and Huh7 hepatocellular carcinoma cells without causing any damage to healthy cells. Thus, AHP-3a may be a natural antioxidant and anticancer component.
Funder
National Natural Science Foundation of China
Hainan Province Clinical Medical Center
Hainan Medical University
Joint Program on Health Science and Technology Innovation of Hainan Province
Reference72 articles.
1. Oxidative stress and Alzheimer’s disease;Zhao;Chin. J. Gerontol.,2013
2. Yuan, Y. (2013, April 01). The Preliminary Research on the Relationship between Oxidative Stress and Allergic Asthma Which Induced by Di-(2-exylhexyl) Phthalate in Ovalbumin-Immunized Mouse Models. Central China Normal University. Available online: https://kns.cnki.net/kns8/Detail?sfield=fn&QueryID=3&CurRec=1&FileName=1013278159.nh&DbName=CMFD201502&DbCode=CMFD.
3. Antioxidant therapy: A new pharmacological approach in shock, inflation, and ischemia/reperfusion injury;Cuzzocrea;Pharmacol. Rev.,2001
4. Tang, J. (2013). Effect of Different Doses of Cyathula Officinalis Polysaccharide on Antioxidant Activity in Mice. [Master’s Thesis, Sichuan Agricultural University].
5. Wang, Y.C. (2022). Study of Clinical and Metabolomics on the Treatment of Non-Small Cell Lung Cancer Based on Pathogenesis Theory of Cancerous Toxin. [Ph.D. Thesis, Nanjing University of Chinese Medicine].