Extraction of Polysaccharides from Root of Pseudostellaria heterophylla (Miq.) Pax. and the Effects of Ultrasound Treatment on Its Properties and Antioxidant and Immune Activities
-
Published:2023-12-26
Issue:1
Volume:29
Page:142
-
ISSN:1420-3049
-
Container-title:Molecules
-
language:en
-
Short-container-title:Molecules
Author:
Li Hangyu12ORCID, Liu Ziwei12ORCID, Liu Qianqian12, Zhang Xinnan12, Li Sheng12, Tang Feng12, Zhang Linzi12, Yang Qian12, Wang Qiran12, Yang Shuyao12, Huang Ling12, Ba Yuwei12, Du Xihui12, Yang Falong12, Feng Haibo12
Affiliation:
1. College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China 2. Key Laboratory of Ministry of Education and Sichuan Province for Qinghai—Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu 610041, China
Abstract
The hydrophilic polysaccharides (PS) were isolated and purified from the tuberous roots of Pseudostellaria heterophylla. The extraction process of PS from Pesudostellariae radix was optimized by single–factor experiments and orthogonal design. The extract was purified by DEAE cellulose column to obtain the pure polysaccharide PHP. Then PHP was treated with different intensities of sonication to study the effect of sonication on PHP’s characteristics and its biological activity in vitro and in vivo. The results of this study revealed that ultrasound treatment did not significantly change the properties of PHP. Further, with the increase of ultrasound intensity, PHP enhanced the proliferation and phagocytosis of macrophage RAW264.7. Meanwhile, it could also significantly improve the body’s antioxidant activity and immune function. The results of this study demonstrated that PHP has the potential as a food additive with enhanced antioxidant and immune functions, and its biological activities could be enhanced by sonication.
Funder
National Natural Science Foundation of China Sichuan provincial Key R&D projects Southwest Minzu University Double World–Class Project Fundamental Research Funds for the Central Universities
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference36 articles.
1. Chen, J., Pang, W., Shi, W., Yang, B., Kan, Y., He, Z., and Hu, J. (2016). Structural Elucidation of a Novel Polysaccharide from Pseudostellaria heterophylla and Stimulating Glucose Uptake in Cells and Distributing in Rats by Oral. Molecules, 21. 2. Structure of a pectic polysaccharide from Pseudostellaria heterophylla and stimulating insulin secretion of INS–1 cell and distributing in rats by oral;Chen;Int. J. Biol. Macromol.,2018 3. Hua, Y., Hou, Y., Wang, S., Ma, Y., Liu, Z., Zou, L., Liu, X., Luo, Y., and Liu, J. (2016). Comparison of Chemical Compositions in Pseudostellariae radix from Different Cultivated Fields and Germplasms by NMR–Based Metabolomics. Molecules, 21. 4. Effects of phosphorus–modified biochar as a soil amendment on the growth and quality of Pseudostellaria heterophylla;Ng;Sci. Rep.,2022 5. Xu, G.B., Zhu, Q.F., Wang, Z., Zhang, C.L., Yang, X., Zhang, J.J., Wang, F.R., Liu, J., Zhou, M., and Wang, Y.L. (2021). Pseudosterins A–C, Three 1–Ethyl–3–formyl–beta–carbolines from Pseudostellaria heterophylla and Their Cardioprotective Effects. Molecules, 26.
|
|