Ulvophyte Green Algae Caulerpa lentillifera: Metabolites Profile and Antioxidant, Anticancer, Anti-Obesity, and In Vitro Cytotoxicity Properties
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Published:2023-01-31
Issue:3
Volume:28
Page:1365
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ISSN:1420-3049
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Container-title:Molecules
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language:en
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Short-container-title:Molecules
Author:
Nurkolis Fahrul1ORCID, Taslim Nurpudji Astuti2ORCID, Qhabibi Faqrizal Ria3ORCID, Kang Sojin45, Moon Myunghan45, Choi Jinwon45, Choi Min45, Park Moon Nyeo45ORCID, Mayulu Nelly6ORCID, Kim Bonglee45ORCID
Affiliation:
1. Department of Biological Sciences, State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga), Yogyakarta 55281, Indonesia 2. Department of Clinical Nutrition, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia 3. Medical School Department, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia 4. Department of Pathology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea 5. Korean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea 6. Nutrition and Food, Faculty of Medicine, Sam Ratulangi University, Manado 95115, Indonesia
Abstract
Marine algae have excellent bioresource properties with potential nutritional and bioactive therapeutic benefits, but studies regarding Caulerpa lentillifera are limited. This study aims to explore the metabolites profile and the antioxidant, anticancer, anti-obesity, and in vitro cytotoxicity properties of fractionated ethanolic extract of C. lentillifera using two maceration and soxhlet extraction methods. Dried simplicia of C. lentillifera was mashed and extracted in ethanol solvent, concentrated and evaporated, then sequentially partitioned with equal volumes of ethyl acetate and n-Hexane. Six samples were used in this study, consisting of ME (Maceration—Ethanol), MEA (Maceration—Ethyl Acetate), MH (Maceration—n-Hexane), SE (Soxhletation—Ethanol), SEA (Soxhletation—Ethyl Acetate), and SH (Soxhletation—n-Hexane). Non-targeted metabolomic profiling was determined using LC-HRMS, while antioxidant, anti-obesity, and anticancer cytotoxicity were determined using DPPH and ABTS, lipase inhibition, and MTT assay, respectively. This study demonstrates that C. lentillifera has several functional metabolites, antioxidant capacity (EC50 MH is very close to EC50 of Trolox), as well as anti-obesity properties (EC50 MH < EC50 orlistat, an inhibitor of lipid hydrolyzing enzymes), which are useful as precursors for new therapeutic approaches in improving obesity-related diseases. More interestingly, ME, MH, and SE are novel bioresource agents for anticancer drugs, especially for hepatoma, breast, colorectal, and leukemia cancers. Finally, C. lentillifera can be a nutraceutical with great therapeutic benefits.
Funder
Basic Science Research Program through the National Research Foundation of Korea National Research Foundation of Korea Korea Health Technology R&D Project through the Korea Health Industry Development Institute
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference40 articles.
1. Seaweeds and microalgae: An overview for unlocking their potential in global aquaculture development;Cai;FAO Fish. Aquac. Circ.,2021 2. Pharmacognosy and In-vivo anticancer potential of an indigenous marine macroalga, Ulvafasciata Delile from Visakhapatnam coast, India;Das;Orient. Pharm. Exp. Med.,2021 3. Comparative production and nutritional value of “sea grapes”—The tropical green seaweeds Caulerpa lentillifera and C. racemosa;Paul;J. Appl. Phycol.,2013 4. Comparative Analysis of Nutrient Composition of Caulerpa lentillifera from Different Regions;Zhang;J. Ocean Univ. China,2019 5. Syakilla, N., George, R., Chye, F.Y., Pindi, W., Mantihal, S., Wahab, N.A., Fadzwi, F.M., Gu, P.H., and Matanjun, P. (2022). A Review on Nutrients, Phytochemicals, and Health Benefits of Green Seaweed, Caulerpa lentillifera. Foods, 11, Available online: https://www.mdpi.com/1827680.
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