Fluorescent-Dye-Labeled Amino Acids for Real-Time Imaging in Arabidopsis thaliana

Abstract

Amino acid is the main transport form of reduced nitrogen in plants. To investigate the uptake and source–sink translocation process of plants to help understand their physiological roles and transport mechanisms, we designed and synthesized three fluorescent-dye-labeled amino acids as tools to visualize amino acid transportation in Arabidopsis thaliana; these amino acids consist of amino acids linked to the fluorophore nitrobenzoxadiazole (NBD) with excellent optical properties. Furthermore, we incubated Arabidopsis thaliana with these NBD fluorescent-dye-labeled amino acids for real-time imaging along with fluorescence enhancement for 24 h. The results showed that Arabidopsis thaliana could absorb them directly from the roots to the leaves. Therefore, our fluorescent-dye-labeled amino acids provide a de novo tool and strategy for visualizing amino acid absorption and transportation in plants.