Sample Preparation Techniques for Growth-Promoting Agents in Various Mammalian Specimen Preceding MS-Analytics

Author:

Klöppner Laura1,Harps Lukas Corbinian1,Parr Maria Kristina1ORCID

Affiliation:

1. Institute of Pharmacy, Freie Universität Berlin, Königin-Luise-Straße 2+4, 14195 Berlin, Germany

Abstract

The misuse of growth-promoting drugs such as beta-2 agonists and steroids is a known problem in farming and sports competitions. Prior to the analysis of biological samples via liquid chromatography (LC)–mass spectrometry (MS) or gas chromatography (GC)–MS, sufficient sample preparation is required to reliably identify or determine the residues of drugs. In practice, broad screening methods are often used to save time and analyze as many compounds as possible. This review was conceptualized to analyze the literature from 2018 until October 2023 for sample preparation procedures applied to animal specimens before LC- or GC-MS analysis. The animals were either used in farming or sports. In the present review, solid phase extraction (SPE) was observed as the dominant sample clean-up technique for beta-2 agonists and steroids, followed by protein precipitation. For the extraction of beta-2 agonists, mixed-mode cation exchanger-based SPE phases were preferably applied, while for the steroids, various types of SPE materials were reported. Furthermore, dispersive SPE-based QuEChERs were utilized. Combinatory use of SPE and liquid–liquid extraction (LLE) was observed to cover further drug classes in addition to beta-2 agonists in broader screening methods.

Funder

State of Berlin, Germany

OpenAccess Publication Fund of Freie Universität Berlin

Publisher

MDPI AG

Reference78 articles.

1. (2023, November 03). Nationale Berichterstattung an die EU 2020, Jahresbericht 2020 zum Nationalen Rückstandskontrollplan (NRKP) und Einfuhrüberwachungsplan (EÜP). Available online: https://www.bvl.bund.de/SharedDocs/Berichte/05_Weitere_Berichte_LM_Sicherheit/Berichte_zur_Lebensmittelsicherheit_2020.pdf?__blob=publicationFile&v=3.

2. Natural estrogens in surface waters of a catchment with intensive livestock farming in Switzerland;Rechsteiner;Environ. Sci. Process. Impacts,2020

3. Medicated livestock carcasses and landfill sites: Sources of highly toxic veterinary pharmaceuticals and caffeine for avian scavengers;Taggart;J. Hazard. Mater.,2023

4. Monitoring estrogen and androgen residues from livestock farms in Phayao Lake, Thailand;Sutaswiriya;Environ. Monit. Assess.,2021

5. Zheng, W., Yoo, K.H., Choi, J.M., Park, D.H., Kim, S.K., Kang, Y.S., Abd El-Aty, A.M., Hacımüftüoğlu, A., Wang, J., and Shim, J.H. (2019). Residual detection of naproxen, methyltestosterone and 17α-hydroxyprogesterone caproate in aquatic products by simple liquid-liquid extraction method coupled with liquid chromatography-tandem mass spectrometry. Biomed. Chromatogr., 33.

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