Emodin-8-O-Glucoside—Isolation and the Screening of the Anticancer Potential against the Nervous System Tumors
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Published:2023-10-31
Issue:21
Volume:28
Page:7366
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ISSN:1420-3049
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Container-title:Molecules
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language:en
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Short-container-title:Molecules
Author:
Okon Estera1, Koval Maryna2ORCID, Wawruszak Anna1ORCID, Slawinska-Brych Adrianna3, Smolinska Katarzyna4, Shevera Myroslav5, Stepulak Andrzej1ORCID, Kukula-Koch Wirginia2ORCID
Affiliation:
1. Department of Biochemistry and Molecular Biology, Medical University of Lublin, 20-093 Lublin, Poland 2. Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Lublin, 20-093 Lublin, Poland 3. Department of Cell Biology, Maria Curie-Sklodowska University, 20-031 Lublin, Poland 4. Chronic Wounds Laboratory, Medical University of Lublin, 20-093 Lublin, Poland 5. M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, 2, Tereshchenkivska Str., 010601 Kyiv, Ukraine
Abstract
Emodin-8-O-glucoside (E-8-O-G) is a glycosylated derivative of emodin that exhibits numerous biological activities, including immunomodulatory, anti-inflammatory, antioxidant, hepatoprotective, or anticancer activities. However, there are no reports on the activity of E-8-O-G against cancers of the nervous system. Therefore, the aim of the study was to investigate the antiproliferative and cytotoxic effect of E-8-O-G in the SK-N-AS neuroblastoma, T98G human glioblastoma, and C6 mouse glioblastoma cancer cells. As a source of E-8-O-G the methanolic extract from the aerial parts of Reynoutria japonica Houtt. (Polygonaceae) was used. Thanks to the application of centrifugal partition chromatography (CPC) operated in the descending mode using a mixture of petroleum ether:ethyl acetate:methanol:water (4:5:4:5 v/v/v/v) and a subsequent purification with preparative HPLC, E-8-O-G was obtained in high purity in a sufficient quantity for the bioactivity tests. Assessment of the cancer cell viability and proliferation were performed with the MTT (3-(bromide 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium), CTG (CellTiter-Glo®) and BrdU (5-bromo-2′-deoxyuridine) assays, respectively. E-8-O-G inhibits the viability and proliferation of SK-N-AS neuroblastoma, T98G human glioblastoma multiforme, and C6 mouse glioblastoma cells dose-dependently. E-8-O-G seems to be a promising natural antitumor compound in the therapy of nervous system tumors.
Funder
Medical University of Lublin
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
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